NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.

100 count, 200 uL max volume per well

Labware definition is available for immediate use in Opentron’s Labware Library

NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.

100 count, 200 uL max volume per well

Labware definition is available for immediate use in Opentron’s Labware Library

Introduction

Usages:
For isolating , cultivating mold and yeast .

Principle:
Potato flour leaching contribute various mold growth; glucose to provide energy; agar as medium coagulant; chloramphenicol inhibit the growth of bacteria.

Formulation(per liter):
Infusion from potatoes 200g
Glucose 20g
Agar 15g
Final pH 5.6 ± 0.2

How to use:
1.Suspend 40g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

Introduction

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

This method is suitable for small quantities of tissue (<100mg) and cells (<5 X10⁶), and large quantities of tissue (up to 1g) and cells (<10⁸), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.

Details

Specifications

Features	Specifications
Main Functions	RNA isolation solvent (substitution for Trizol/Qiazol reagent)
Applications	RT-PCR, Northern hybridization, poly (a) enrichment, etc.
Purification technology	Acid phenol guanidine isothiocyanate
Process method	Manual (centrifugation)
Sample type	Animal tissue, plant tissue, adherent cells, suspended cells, bacteria, trace fungi, etc.
Sample amount	Flexible
Elution volume	Variation with sample size
Time per run	Variation with sample size

Advantages

• Extensive – suitable for various kinds of biological samples, including animals, plants, cultured cells, bacteria, etc.
• High yield – efficient cleavage releases more RNA
• High purity – purified RNA is suitable for various downstream applications
• Flexible – sample amount can be adjusted according to the demand
• High cost performance compared with similar products

Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.

Experiment Data

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.