DBCO-PEG1-amine TFA salt

Description 

The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users. 

Features

• Cloning efficiency greater than 90% 
• IPTG and X-Gal are not required 
• Accepts a wide range of insert/vector ratios 0.5:1 to 12:1 
• Accepts insert size from 6 bp to 11 kb
• The phosphorylation of PCR fragments is not required
• Accepts blunt end amplicon or DNA fragment (not for sticky ends)
• Ampicillin and kanamycin selection markers

Storage

-20°C for 24 months

The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users.

DBCO-PEG4-PABA is an analog of DBCO-Acid with PEG linker and a 4-Aminobenzoic acid (PABA) group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. Reagent grade, for research use only.

DBCO-PEG4-PABA is an analog of DBCO-Acid with PEG linker and a 4-Aminobenzoic acid (PABA) group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. Reagent grade, for research use only.

K-RINTDF

SKU: 700004335

100 assays per kit

The Rapid Integrated Total Dietary Fiber Assay Kit method is validated under collaborative study (AACC Method 32-60.01, AOAC Method 2022.01, AOAC Method 2017.16, ICC Standard No. 185) and is recognized as a Type I Method by CODEX Alimentarius. The K-RINTDF method is the recommended one for the measurement of total dietary fiber in all foods that may or may not contain resistant starch. This method is updated to be more consistent with in vivo conditions in the human small intestine, i.e. a 4 h incubation time. Under these conditions more accurate measurement of resistant starch is obtained, including phosphate cross-liked starch (RS₄). Use of higher enzyme concentrations ensures that resistant maltodextrins produced from non-resistant starch under the incubation conditions of the Integrated Total Dietary Fiber procedure (AOAC Methods 2009.01 and 2011.25) are no longer produced.

In this improved, rapid method, the incubation time with PAA + AMG is reduced to 4 h and the levels of both PAA and AMG are increased to ensure that resistant starch levels obtained with a set of control samples are consistent with ileostomy data. Under these conditions, the DF values obtained for most samples are the same as those obtained with AOAC Methods 2009.01 and 2011.25.

The dietary fiber fractions that are measured with this method are:

1. High Molecular Weight Dietary Fiber (HMWDF) including Insoluble Dietary Fiber (IDF) and High Molecular Weight Soluble Dietary Fiber (SDFP; soluble dietary fiber which is precipitated in the presence of 78% aqueous ethanol), and

2. Low Molecular Weight Soluble Dietary Fiber (SDFS; water soluble dietary fiber that is soluble in the presence of 78% aqueous ethanol).

Alternatively, IDF, SDFP and SDFS can be measured separately.

The enzymes used in this method are high purity and effectively devoid of contaminating enzymes active on other dietary fiber components such as β-glucan, pectin and arabinoxylan. They are supplied as freeze-dried powders; allowing the use of glycerol as an internal standard in the method.

See our full range of dietary fiber assay kits.

View Dietary Fiber Measurement Guide – Which Method for which sample?

* See McCleary, B. V., Sloane, N & Draga, A. (2015). Determination of total dietary fibre and available carbohydrates: a rapid integrated procedure that simulates in vivo digestion. Starch/Starke, 66, 1-24.

Validation of Methods

Advantages

• More rapid measurement – incubation time with PAA + AMG reduced to 4 h in comparison with AOAC 2009.01 (increased levels of enzyme employed) 
• DF values for most samples are very similar to those obtained with AOAC Method 2009.01 
• Rapid Integrated Total Dietary Fiber method removes all of the limitations that have been identified with AOAC Method 2009.01* 
• All reagents stable for > 2 years after preparation 
• The method is consistent with the CODEX Alimentarius definition of dietary fiber 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Very competitive price (cost per test)

The Rapid Integrated Total Dietary Fiber Assay Kit method is validated under collaborative study (AACC Method 32-60.01, AOAC Method 2022.01, AOAC Method 2017.16, ICC Standard No. 185) and is recognized as a Type I Method by CODEX Alimentarius. The K-RINTDF method is the recommended one for the measurement of total dietary fiber in all foods that may or may not contain resistant starch. This method is updated to be more consistent with in vivo conditions in the human small intestine, i.e. a 4 h incubation time. Under these conditions more accurate measurement of resistant starch is obtained, including phosphate cross-liked starch (RS4). Use of higher enzyme concentrations ensures that resistant maltodextrins produced from non-resistant starch under the incubation conditions of the Integrated Total Dietary Fiber procedure (AOAC Methods 2009.01 and 2011.25) are no longer produced.