t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane is a branched crosslinker molecule with three terminal propargyl groups and a t-Boc protected amide group. The propargyl groups can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected amine can be deprotected under acidic conditions. Reagent grade, for research use only.

t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane is a branched crosslinker molecule with three terminal propargyl groups and a t-Boc protected amide group. The propargyl groups can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected amine can be deprotected under acidic conditions. Reagent grade, for research use only.

Introduction

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 3g plant and fungal tissue
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Various plant samples (including conventional, polysaccharides and polyphenols)
Sample amount	Fresh / frozen plant samples: 2-3 gDried plant / seed samples: 0.5-1 g
Elution volume	≥500μl
Time per run	≤120 minutes
Liquid carrying volume per column	20ml
Binding yield of column	5mg

Principle

This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

• Broad spectrum – suitable for extracting DNA from various plant samples
• Fast – several samples can be extracted in 30 minutes by silica technology
• High purity – high quality DNA, completely remove inhibitors
• High yield – silica technology can achieve the highest yield

Kit Contents

Contents	D316302	D316303
Purification Times	10 Preps	50 Preps
RNase A	20 mg	90 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer PAL	180 ml	900 ml
Buffer GWP	150 ml	800 ml
Buffer GW2	25 ml	200 ml
Buffer AE	30 ml	120 ml
HiPure DNA Maxi Columns II	10	50
50 ml Collection Tubes	20	100

Storage and Stability

RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from bacterial culture
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Gram negative or positive bacteria
Sample amount	Bacterial culture: 0.5-1.5ml
Elution volume	≥60μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Fast – several samples can be extracted in 60 minutes (after digestion)
• High purity – unique adsorbent can completely remove inhibitory factors
• High recovery – DNA can be recovered at the level of PG

Kit Contents

Contents	D513101	D513102	D513103
Purification Times	48 Preps	96 Preps	480 Preps
MagPure Particles	1.2 ml	2.5 ml	11 ml
Lysozyme	60 mg	120 mg	600 mg
Proteinase K	24 mg	50 mg	220 mg
Protease Dissolve Buffer	3 ml	6 ml	30 ml
Buffer SDS	1.5 ml	3 ml	15 ml
Buffer MLA	30 ml	60 ml	300 ml
Buffer GW1*	13 ml	44 ml	110 ml
Buffer TE	30 ml	40 ml	200 ml

Storage and Stability

MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.