DBCO-PEG2-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG2-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
R4316 HiPure Circulating DNA/RNA Kit
Product Info
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Product Info
Introduction
This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection. HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
Details
Specifications
Features
Specifications
Main Functions
Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma
Applications
qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method
Midi spin column
Purification technology
Silica technology, DNA filtration technology
Process method
Manual (centrifugation or vacuum)
Sample type
serum, plasma, and other cell-free liquid samples
Sample amount
1-5 ml
Elution volume
≥20μl
Time per run
≤100 minutes
Liquid carrying volume per column
4 ml
Binding yield of column
1 mg
Principle
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finally, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.
Advantages
High yield – most optimized process to obtain maximum free RNA and small RNA
High concentration – low elution volume (>20μl) to ensure nucleic acid concentration
High purity – low alcohol combination, completely remove inhibitor and protein pollution
High recovery – silica gel column technology can recover nucleic acid molecules at the level of PG
Large volume – 1-2ml serum and plasma samples can be processed at one time
Kit Contents
Contents
R431602
D431603
Purification Times
50 Preps
250 Preps
HiPure RNA Micro Columns
50
5 x 50
HiPure Viral Midi Columns
50
5 x 50
15 ml Collection Tubes
50
5 x 50
2ml Collection Tubes
50
5 x 50
Buffer CFL
150 ml
2 x 375 ml
Buffer CFP
30 ml
150 ml
Buffer MGW1*
100 ml
2 x 250 ml
Buffer RW2*
2 x 50 ml
5 x 100 ml
RNase Free Water
10 ml
50 ml
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
This product is a Interleukin-6 Quality Control (two areas) for Milenia QuickLine IL-6 Test (MQL6 1).
This product is only available within the EU!
Method/Platform
control
Range/Assay Sensivity
two areas
Test Principle
The controls are designed only for internal Quality Control of Milenia QuickLine Il-6 Tests. Test results can only be evaluated with Milenia POCScan Reader.
Brief Instructions
Storage
2°C to 8°C
Components
IL-6 Control 1, IL-6 Control 2
Document
2 x 3 flasks
This product is a Interleukin-6 Quality Control (two areas) for Milenia QuickLine IL-6 Test (MQL6 1).
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 & 150 reactions