DBCO-PEG2-PFP ester is a PEG active ester consisting of a DBCO group which can react with azides under copper free conditions. The PFP ester is an active ester which can react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG2-PFP ester is a PEG active ester consisting of a DBCO group which can react with azides under copper free conditions. The PFP ester is an active ester which can react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Product Description
One-Step Isothermal Amplification Basic Kit for High Throughput RNA Analysis
Kit Storage and Term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal Nucleic Acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit Basic |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal Nucleic Acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal Nucleic Acid Applications
Suitable for RNA isothermal rapid amplification kit(BASIC type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
K-URONIC
SKU: 700004354
100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser)
| Content: | 100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 1 year under recommended storage conditions |
| Analyte: | D-Galacturonic Acid, D-Glucuronic Acid |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 5 to 150 µg of D-glucuronic acid or D-galacturonic acid per assay |
| Limit of Detection: | ~ 15.5 mg/L |
| Reaction Time (min): | ~ 10 min at 25oC or ~ 5 min at 37oC |
| Application examples: | Hydrolysates of plant material and polysaccharides and other materials. |
| Method recognition: | Novel method |
The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our complete range of mono/disaccharide test kits.
Advantages
The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.
Features:
Large capacity water capture freeze dryer
Tabletop medium-sized freeze dryer
Suitable for drying medium-sized samples
Apply to drying in laboratories such as cosmetics, fermentation broth, soil, materials, etc.
product description:
9kg-12kgs water capture capacity, which can meet the needs of laboratories with large freeze-drying requirements for water capture capacity
-70°C/-90°C ultra-low temperature cold trap, which can capture lower temperature organic solvents
Unique refrigeration technology, the cooling time of the cold trap from the start of the low-temperature refrigeration system to the target value is ≤3 minutes
Direct-capture large coil cold trap, higher efficiency, better ice capture effect
Hemispherical cold trap bottom surface is more conducive to the discharge of condensed water
The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry a large amount of ultra-low temperature organic solvents
One-click hot gas defrost, with defrost compressor protection program.
Large flat flange silicone seal, permanently no need to apply any auxiliary sealing silicone grease
High-precision ultra-wide width detection, vacuum sensor with full-range automatic temperature compensation, making vacuum display more accurate
The cavity vacuum can be read and controlled accurately, and the vacuum control accuracy is ≤0.1mbar
Removable sample shelf with dedicated holes for centrifuge tube placement, making placement and height adjustment easier
Configuration of various types of chambers and T-frames can fully satisfy users’ sample placement
Optional configuration of eutectic point detection system and data export system.
The vacuum pump and sample placement chamber can be combined at will according to user needs, making the configuration more flexible and convenient
Techinical parameter
9-12L Floor tabletop and floor standing one for lab usage
| Tabletop | BD1-9 | BD2-9 | BD1-12 | BD2-12 |
| Floor standing | BH1-9 | BH2-9 | BH1-12 | BH2-12 |
| Water Capture capacity | 9L | 12L | ||
| Cold trap temperature | -70 C | -90℃ | -70C° | -90℃ |
| Cold trap volume | 15L | 20L | ||
| Freeze dried chamber | High permeability organic glass/multi manifold external valve/stainless steel chamber | |||
| Sample shelf | Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional | |||
| Vacuum pump | Extreme vacuum 4 * 10-4mbar, pumping capacity ≥ 200L/can be equipped with various types of pumps | |||
| Oil mist filter | Standard configuration | |||
| Vacuum pump connection pipe | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.5m | |||
| anti-corrosive treatment | Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents | |||
| External valve | 1-48 ports/optional | |||
| Eutectic point judgment | Support/Optional | |||
| Data audit trail | Support/Optional | |||
| Power | 0.9kw | 1.5kw | 1kw | 1.6KW |
| Size | 390*468*448mm | 480*498*487mm | ||
