DBCO-PEG2-PFP ester is a PEG active ester consisting of a DBCO group which can react with azides under copper free conditions. The PFP ester is an active ester which can react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG2-PFP ester is a PEG active ester consisting of a DBCO group which can react with azides under copper free conditions. The PFP ester is an active ester which can react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.
Q-PAGE™ TGN Precast Gels are available in gradient (4 to 15%) and fixed (10%) concentrations of polyacrylamide in 12- and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP4XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP5XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.
Q-PAGE™ TGN Precast Gels are available in gradient (4 to 15%) and fixed (10%) concentrations of polyacrylamide in 12- and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP4XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP5XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
For selective enrichment culture of Listeria monocytogenes in food.
Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; esculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.
| Ingredients | /liter |
| Enzymatic digest of animal tissues ( Proteose peptone) | 5.0g |
| Enzymatic digest of casein ( Tryptone) | 5.0g |
| Meat extract | 5.0g |
| Yeast extract | 5.0g |
| Sodium chloride | 20.0g |
| Disodium hydrogen phosphate dihydrate | 12.0g |
| Potassium dihydrogen phosphate | 1.35g |
| Aesculin | 1.0g |
| Lithium chloride | 3.0g |
| pH7.2±0.2 at 25°C | |
Suspend 57.4g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃,aseptically add the contents of 1 vial of Fraser Selective Supplement (SR0120) to each 225 mL of base to prepare FB1 solution; Add one reagent (SR0130) to each100 mL of FB2 culture medium to prepare FB2 enrichment solution.
Cultural characteristics observed after incubation at 35-37°C for 46~50 hours
| Quality control strains | Growth | Characteristics |
| Listeria monocytogenes ATCC19115 + Escherichia coli ATCC25922 + Enterococcus faecalis ATCC29212 | >20 cfu On PALCAM | Gray to black colony count with black halo |
| Escherichia coli ATCC25922 | < 100 colonies on TSA | Inhibition |
| Enterococcus faecalis ATCC29212 |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
On June 14, 2024
ISO 11290
Intended Use For selective enrichment culture of Listeria monocytogenes in food. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen ……
Usages:
For cultivations of yeasts , moulds and acidophilic bacteria.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.
Formulation(per liter):
Peptone 5g
Tryptone 5g
Glucose 20g
Final pH 5.7 ± 0.1
How to use:
1.Suspend 30g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Quality control:
| Item | The name and number of strain | Growth | Colony Color |
| 1 | Aspergillus niger ATCC16404 | Good | Spore surface growth |
| 2 | Candida albicans ATCC10231 | Good | Cloudy broth |
| 3 | Escherichia coli ATCC25922 | Inhibition | — |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
