DBCO-PEG23-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG23-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-amine TFA salt is a simple building block containing a DBCO moiety and an amine group. Amine is very reactive with NHS ester. DBCO is commonly used for copper-free Click Chemistry reactions.
DBCO-amine TFA salt is a simple building block containing a DBCO moiety and an amine group. Amine is very reactive with NHS ester. DBCO is commonly used for copper-free Click Chemistry reactions.
Intended Use
For the cultivation of E. coli in fermentation and molecular genetic studies.
Principle and Interpretation
Peptone provides nitrogen . Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Glucose provides carbon.Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent.
Formulation
| Ingredients | /liter |
| Peptone | 10.0g |
| Yeast extract | 5.0g |
| Sodium chloride | 5.0g |
| Glucose | 1.0g |
| Agar | 15.0g |
| pH7.0±0.2 at 25°C | |
Preparation
Suspend 36g in 1 litre of distilled water. Bring to a boil to dissolve completely. Sterilize by autoclaving at 121°C for 15minutes.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24 hours
| Quality control strains | Growth |
| Escherichia coli ATCC25922 | good |
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
