Description

30nm Colloidal Gold for Lateral Flow is a highly stable and uniform 30 nm gold nanoparticles can be supplied in a range from 1 OD to 100 OD. The quality and performance of a conjugate is critical to successful lateral flow test manufacturing. Our products are made in Austin TX and produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.

Bulk pricing and manufacturing supply contracts available. 

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Functionally Tested in Lateral Flow

Specifications 1OD 30nm Gold (1L)

Number of particles/mL	1.3-2 x 1011
Gold Concentration (mg/mL)	4.2-4.7 x 10-2
Molar Concentration (moles/liter)	2.2-3.3 x 10-10
Particle Diameter	30 nm /-1.5

Number of particles/mL 1.3-2 x 1011
• Gold Concentration (mg/mL) 4.2-4.7 x 10-2
• Molar Concentration (moles/liter) 2.2-3.3 x 10-10
• Particle Diameter 30 nm /-1.5

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from bacterial culture
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Gram negative or positive bacteria
Sample amount	Bacterial culture: 0.5-1.5ml
Elution volume	≥60μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Fast – several samples can be extracted in 60 minutes (after digestion)
• High purity – unique adsorbent can completely remove inhibitory factors
• High recovery – DNA can be recovered at the level of PG

Kit Contents

Contents	D513101	D513102	D513103
Purification Times	48 Preps	96 Preps	480 Preps
MagPure Particles	1.2 ml	2.5 ml	11 ml
Lysozyme	60 mg	120 mg	600 mg
Proteinase K	24 mg	50 mg	220 mg
Protease Dissolve Buffer	3 ml	6 ml	30 ml
Buffer SDS	1.5 ml	3 ml	15 ml
Buffer MLA	30 ml	60 ml	300 ml
Buffer GW1*	13 ml	44 ml	110 ml
Buffer TE	30 ml	40 ml	200 ml

Storage and Stability

MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.

K-RAFGA

SKU: 700004331

120 assays per kit

Content:	120 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	D-Galactose, Raffinose
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 83 µg of D-galactose per assay (i.e. approx. 12 to 250 µg of raffinose per assay)
Limit of Detection:	21 mg/L
Reaction Time (min):	~ 60 min
Application examples:	Cereal flours, soybean flour, by-products of sucrose manufacture and other materials.
Method recognition:	Used and accepted in food analysis

The Raffinose/D-Galactose test kit allows for the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View all of our monosaccharide test kit products.

Advantages

• Very rapid reaction due to inclusion of galactose mutarotase (patented technology) 
• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Raffinose/D-Galactose test kit allows for the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.