DBCO-PEG24-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG24-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Phosphate Assay Kit
Product Info
Document
Product Info
K-PHOS
SKU: 700004326
100 assays (manual) / 400 assays (auto-analyser)
Content:
100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Phosphate
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
360
Signal Response:
Increase
Linear Range:
0.1 to 10 μg of phosphate per assay
Limit of Detection:
0.16 mg/L
Reaction Time (min):
~ 20 min
Application examples:
Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.).
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
This method is suitable for analysis using spectrophotometer and auto-analyser.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.
Need other products? See our complete list of assay kits.
Advantages
Chemically defined detection method
All reagents stable for > 2 years after preparation
Rapid reaction (~ 20 min)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual and auto-analyser formats
Document
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
Propargyl-PEG4-Ms has an alkyne group and a mesylate group. The mesylate group is a sulfonate ester for nucleophilic substitution reactions (SN2). The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environments. Reagent grade, for research use only.
Document
Propargyl-PEG4-Ms has an alkyne group and a mesylate group. The mesylate group is a sulfonate ester for nucleophilic substitution reactions (SN2). The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environments. Reagent grade, for research use only.
DBCO-PEG5-NHS Ester is a DBCO-containing PEG linker containing NHS ester that can react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG5 spacer increases the water solubility of compounds in aqueous media. DBCO is commonly used for copper-free Click Chemistry reaction. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG5-NHS Ester is a DBCO-containing PEG linker containing NHS ester that can react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG5 spacer increases the water solubility of compounds in aqueous media. DBCO is commonly used for copper-free Click Chemistry reaction. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.