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DBCO-PEG24-Maleimide

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DBCO-PEG24-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Detail

DBCO-PEG24-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Other Products

C13130 HiPure DNA Plate

Introduction

Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.

The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.

Details

Specifications

FeaturesSpecifications
Recommended applicationSmall amounts of nucleic acid isolation, viral nucleic acid from cell free samples
Preservation conditionsRoom temperature
StabilityUp to 4 years
Filter membraneHigh quality glass fiber filter GF/F, 2 layers
Membrane aperture0.7μm
Maximum binding yield of plasmid30 μg
Maximum yield of alcohol mediated Binding100 μg
Single liquid carrying capacity of column900 μl
Minimum elution volume80 μl
Withstand centrifugal force5,000 x g
CentrifugeLow speed centrifuge, Swing out Rotor, can placed a height of 6.5cm square,  (height of HiPure DNA Plate & 1.6ml Collection Plate: height, 6.2cm)

Adsorption Mechanism

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.

Ordering information

CAT.No.Product NamePackage
C13130HiPure DNA Plate (2 x GF/F)with 1.6ml Collection Plate10/Bag

Purchase Guide

Item No.Product NameMembrane type/number of layersCollection tubesPlasmid DNA binding capacity (Physical adsorption)gDNA/RNA binding capacity (Alcohol-mediated adsorption)Minimum Elution volumeLiquid volume capacity
C13010HiPure DNA Nano Column2 layers GF/F2ml without cap5μg20μg10μl700μl
C13011HiPure DNA Micro Column3 layers GF/F2ml without cap10μg50μg15μl700μl
C13100HiPure DNA Mini Column I2 layers GF/B2ml without cap15μg100μg30μl700μl
C13110HiPure DNA Mini Column II4 layers GF/B2ml without cap35μg200μg50μl800μl
C13111HiPure RNA Mini Column3 layers GF/B2ml without cap30μg200μg30μl800μl
C13112HiPure Viral Mini Column3 layers GF/F2ml without cap30μg200μg30μl800μl
C13113HiPure CFDNA Mini Column3 layers GF/F,1 layer GF/B2ml without cap30μg200μg30μl800μl
C13120HiPure DNA Midi Column4 layers GF/B15ml Centrifuge tube125μg1mg500μl4ml
C13121HiPure DNA Midi Column III8 layers GF/B15ml Centrifuge tube250μg1mg500μl4ml
C13122HiPure DNA Maxi Column4 layers GF/B50ml Centrifuge tube500μg5mg1000μl20ml
C13123HiPure DNA Maxi Column III8 layers GF/B50ml Centrifuge tube1mg5mg1000μl20ml
C13124HiPure DNA Maxi Column C8 layers GF/B50ml high speed Centrifuge tube1mg5mg700μl12ml
C13130HiPure DNA Plate2 layers GF/F1.6ml Plate30μg100μg80μl900μl
C13131HiPure gDNA Plate2 layers GF/B1.6ml Plate30μg100μg80μl900μl

Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.

endo-BCN-PEG8-acid

endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Alkaline Phosphatase Assay Kit

Highly Sensitive, rapid, robust screening kit for sample phosphatase activity.

Description

For plate-based colorimetric enzymatic determination of alkaline phosphatase:

Distributed in almost every tissue of the body, serum alkaline phosphatase (ALP) levels are of interest in the testing for hepatobiliary disorder and bone disease. Most of the ALP in the normal adult serum is from the liver or biliary tract. Normal alkaline phosphatase levels are age-dependent and are elevated during periods of active bone growth. Moderate elevations of ALP (not involving the liver or bone) may be attributed to Hodgkin’s disease, congestive heart failure, and abdominal bacterial infections.

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma, and sarcoidosis.

The kit contains sufficient materials to rapidly test 42 samples in duplicate.