Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions

OverView

ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.

Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.

‍

Key Features 

• Easy to inactivate after use: 15 – 30 min at 55 – 60°C 
• Broad substrate specificity
• Active at high salt concentrations
• Compatible with downstream DNA analyses
• Available in Glycerol FREE formulation

Applications

• Lysis and releasing nucleic acid from single-cells and small tissue samples
• Optimization of DNA/RNA yields from nucleic acids when added to extraction procedures

Figures

ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.

Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.

Introduction

This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of blood samples. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids in as little as 25 minutes. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from whole blood using economic salt out method
Applications	PCR, enzyme digestion, Southern hybridization,etc.
Purification technology	Salting out method
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissue
Sample amount	Unlimited
Elution volume	≥100μl
Time per run	Variation with sample amount

Principles

Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.

Advantages

• Safe – without phenol chloroform extraction
• Environment friendly – the reagents used are safe, non-toxic and without pollution
• High molecular weight – the molecular weight of genomic DNA is about 50-150kb
• High purity – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
• Unlimited sample size – solution type operation, sample volume can be adjusted at will
• Cost performance – the most economical nucleic acid extraction technology

Kit Contents

Contents	D331101	D331102	D331103
Purification Volumes	50 ml	300 ml	1000 ml
10 x Buffer RBC	20 ml	100 ml	3 x 100 ml
Buffer WTL	60 ml	350 ml	1000 ml
Buffer PPS	20 ml	120 ml	350 ml
RNase Solution	300 µl	1.8 ml	6 ml
Buffer TE	10 ml	30 ml	120 ml

Storage and Stability

RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of blood samples. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids in as little as 25 minutes. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.