DBCO-PEG24-TFP ester is a heterobifunctional PEG linker with an azide-reactive DBCO and an amine-reactive TFP ester that is less susceptible to hydrolysis than an NHS ester. The PEG linker improves the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG24-TFP ester is a heterobifunctional PEG linker with an azide-reactive DBCO and an amine-reactive TFP ester that is less susceptible to hydrolysis than an NHS ester. The PEG linker improves the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Attogene’s Lead Rapid Lab Lateral Flow Kit can be used for the screening of Lead in water and food samples at greater than or equal to 10 ppb in a laboratory setting. Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.
Lead contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Lead ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking Lead-contaminated food and water include brain, heart, kidney, lungs and immune system problems for adults, and the physical and mental development delays in infants and children. Attogene’s Lead Lateral Flow test gives results conforming of 10ppb or greater in 5-15 minutes.
This is a revolutionary product designed to make Lead testing in easy and affordable. This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L).
Sample Collection:
1. Take a first-draw sample
Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.
2. Take a flush sample
If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.
3. Take sequential samples
If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.
Standards and Regulations and recommendations for Lead
High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death. Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.
Document
Screening of Lead in water samples at 5-10 ppb Format: 10 tests (5 tests/5 controls) Run Time: 15 Minutes
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from <10 mg insect tissue
Applications
PCR, southern bolt and virus detection, etc
Purification method
Midi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Insect tissue samples
Sample amount
<10 mg
Elution volume
≥15μl
Time per run
≤30 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
Fast – several samples can be extracted in less than 30 minutes
Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
Safe – without phenol chloroform extraction and alcohol precipitation
Kit Contents
Contents
D312902
D312903
Purification Times
50
250
HiPure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Buffer ITL
30 ml
120 ml
Buffer IL*
30 ml
120 ml
Buffer GW1*
22 ml
110 ml
Buffer GW2*
20 ml
2 x 50 ml
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
15 ml
Buffer AE
15 ml
60 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
Document
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
Document
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.