DBCO-PEG24-TFP ester is a heterobifunctional PEG linker with an azide-reactive DBCO and an amine-reactive TFP ester that is less susceptible to hydrolysis than an NHS ester. The PEG linker improves the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG24-TFP ester is a heterobifunctional PEG linker with an azide-reactive DBCO and an amine-reactive TFP ester that is less susceptible to hydrolysis than an NHS ester. The PEG linker improves the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ TGN Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.
Features
Contents
0.025 M Tris, 0.192 M glycine, 0.10% SDS
Applications
Running buffer for Laemmli Tris-HCl gel electrophoresis
Storage
Room temperature for 24 months
1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ TGN Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.
Sample type purification kit guide
The 16S V2-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V2-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V2-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V2-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V2-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70300 (24 preps) | Cat. 70310, 70320, 70330, 70340 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V2-V3 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Specifications
| Features | Specifications |
| Main Functions | Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Serum, plasma |
| Sample amount | 0.6ml |
| Elution volume | |
| Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA
| Contents | 1292750 | 12927200 |
| Purification Times | 50 preps | 200 preps |
| MagPure Particles F | 1.2 ml | 4.5 ml |
| MagBind Particles | 1.2 ml | 4.5 ml |
| Proteinase K | 24 mg | 90 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer MLB | 30 ml | 120 ml |
| Buffer GDP | 15 ml | 60 ml |
| Buffer MAW1 | 40 ml | 250 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 60 ml |
Storage and Stability
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
