DBCO-PEG4-biotin is a click chemistry biotinylation that can enable copper-free click chemistry with azide molecules to form a stable triazole linkage. PEG4 spacer increases aqueou solubility of the molecules conjugated to the biotin compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-biotin is a click chemistry biotinylation that can enable copper-free click chemistry with azide molecules to form a stable triazole linkage. PEG4 spacer increases aqueou solubility of the molecules conjugated to the biotin compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Specifications
| Features | Specifications |
| Concentration | 100 mg/ml |
| Appearance | Suspension of black particles |
| Surface functional group | Si-OH, Silanol |
| Dispersibility | Polydisperse Amorphous |
| Particle size | 1.5-5 μm |
| Preservation conditions | Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth. |
| Magnetic response speed | 15-30 seconds |
| Settling velocity | >5 minutes |
| High salt mediated binding | >2M guanidine isothiocyanate, DNA recovery up to 80% |
| Alcohol mediated binding | 2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
| PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
| DNase/RNase | Not detected |
| DNA residue | <1 ppm |
| Recommended application | Plasmid extraction, gel DNA recovery, genomic DNA extraction and RNA extraction. |
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
Ordering information
| CAT.No. | Product Name | Package |
| C14100 | MagPure Particles | 100 ml |
| C14101 | MagPure Particles | 400 ml |
| C14102 | MagPure Particles | 3 x 400 ml |
| C14103 | MagPure Particles | 10 x 400 ml |
| Features | MagPure Particles | MagPure Particles N | MagPure Particles G | MagPure Particles F | MagBind Particles |
| Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
| Concentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
| Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
| Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
| Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
| Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
| Color | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
| Magnetic response | 15-30s | ~60s | ~30s | 20s | 120s |
| Settling Time (1ml) | >5min | >10min | >3min | >3min | >2h |
| Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
| DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
| DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
| Recommended Use | gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification | DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up | Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation | Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction | DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays |
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
TXK4 Blood card centrifuge, ID card Centrifuge, Gel card centrifuge
Application:
TXK4 blood group serological tests multipurpose centrifuge widely used in the test of blood group serology, blood type, Microcolumn agglutination, immunodetection and etc.
Features:
1. Brushless motor to make sure free maintenance,no powder polution, micro computer control and digital display the time, speed and RCF to let operation easily.
2. Special centrifugation technical to different reagent according to the requirement.
3. Regular centrifugation program, customers can press the button to choose the required one.
4. Electrical lid lock, over speed and imbalance protection.
Technical Parameters:
| Max speed | 4000rpm | Max RCF | 2250xg |
| Max volume | 24 cards | Noise | ≤45dBA |
| Timer | 0～99min | Net weight | 23KG |
| Dimension(HxDxW) | 483×320×265mm | Power supply | AC 220 50HZ 2A |
| Speed accuracy | ±10rpm | Package | carton box |
Matched Rotors for TXK4:
| Order No. | Rotor Type | Max Speed (rpm) | Max Volume (ml) | Max RCF(g) |
| NO33183 | Swing Rotor | 3000 | 24 cards | 1080 |
| 4000 | 24 cards | 2120 | ||
| NO31605 | Fixed Rotor | 4000 | 30×7/5ml | 2250 |
| NO31601 | Fixed Rotor | 4000 | 12×10ml | 2150 |
TXK4 blood group serological tests multipurpose centrifuge widely used in the test of blood group serology, blood type, Microcolumn agglutination, immunodetection and etc.
