DBCO-PEG4-biotin

t-Boc-N-Amido-PEG7-propargyl is a crosslinking reagent that enables the formation of triazole linkage with azides under the catalyzation of copper. The Boc-protected amine can be deprotected under mild acidic conditions. By introducing PEG chain into the molecule, the hydrophilicity can be greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

t-Boc-N-Amido-PEG7-propargyl is a crosslinking reagent that enables the formation of triazole linkage with azides under the catalyzation of copper. The Boc-protected amine can be deprotected under mild acidic conditions. By introducing PEG chain into the molecule, the hydrophilicity can be greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Fast and easy processing using rapid and convenient spin-columns
• Isolate high quality and high yield DNA
• DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping 

This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids.  The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping.  The protocol can be completed in as little as 1 hour.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity (gDNA)	10 μg
Maximum Loading Volume Per Spin Column	650 μL
Size of DNA Purified	All sizes > 80 bp
Maximum Amount of Starting Material	5 sections < 20 µm thick paraffin slices 25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.

Component	Cat. 47400 (50 preps)
Digestion Buffer A	25 mL
Buffer RL	30 mL
Wash Solution A	38 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
RNase A	1 tube
gDNA Purification Micro Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Propargyl-PEG3-amine is a PEG linker that is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group reacts with azides in copper catalyzed azide-alkyne Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG3-amine is a PEG linker that is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group reacts with azides in copper catalyzed azide-alkyne Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.