DBCO-PEG4-Butyraldehyde is a click chemistry PEG reagent containing a terminal butylraldehyde group. Butyraldehyde reacts with hydrazide to form a stable hydrazone linkage or with alkoxyamines to form a stable oxime bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
DBCO-PEG4-Butyraldehyde is a click chemistry PEG reagent containing a terminal butylraldehyde group. Butyraldehyde reacts with hydrazide to form a stable hydrazone linkage or with alkoxyamines to form a stable oxime bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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UltraRanger 1kb DNA Ladder (Cat# 12100) – 100 loads
Ladder Properties:
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
| Fragment | Size (bp) | Mass (ng) |
| 1 | 24000 | 30 |
| 2 | 14000 | 27 |
| 3 | 10000 | 60 |
| 4 | 8000 | 44 |
| 5 | 6000 | 36 |
| 6 | 5000 | 55 |
| 7 | 4000 | 49 |
| 8 | 3000 | 39 |
| 9 | 2500 | 35 |
| 10 | 2000 | 30 |
| 11 | 1500 | 33 |
| 12 | 1000 | 10 |
| 13 | 700 | 21 |
| 14 | 500 | 18 |
| 15 | 300 | 11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
DBCO-PEG4-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
SKU: MTP96
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit
