DBCO-PEG4-Butyraldehyde is a click chemistry PEG reagent containing a terminal butylraldehyde group. Butyraldehyde reacts with hydrazide to form a stable hydrazone linkage or with alkoxyamines to form a stable oxime bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
DBCO-PEG4-Butyraldehyde is a click chemistry PEG reagent containing a terminal butylraldehyde group. Butyraldehyde reacts with hydrazide to form a stable hydrazone linkage or with alkoxyamines to form a stable oxime bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
Sample type purification kit guide
The 16S V3-V5 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V3-V5 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V3-V5Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70500 (24 preps) | Cat. 70510, 70520, 70530, 70540 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V3-V5 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
Cell Washer/Serofuge Centrifuge
Features
1. Microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.
2. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.
3. Special program, HLA and SERO separately.
5. Micro computer control system, LED display the RCF，time and speed.
6.. Electric lid lock, compact design, super speed and imbalance protection.
TD4 Technical Parameter：
| Max. Speed | 4000rpm |
| Max. RCF | 2000 |
| Max. Capacity | 12×7 |
| Time Range | 0~99min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 55 |
| Temperature | Normal |
| Acc/Dcc | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | / |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 485×320×255mm |
| Net Weight(Kg) | 24KG |
Matched Rotors for TD4
| Order No. | Rotor | Max Speed (rpm) | Max Volume(ml) | Max. RCF（×g） |
| NO31501 | SERO Rotor | 3000 | 12×7/5ml(10-13*60-100mm) | 2000 |
| NO31502 | HLA Rotor | 4000 | 12×2/1.5/0.5ml | 1000 |
| Rotor Name | Max RCF(×g) | Use |
| HLA rotor for Lymphocyte washing | 2000 | Lymphocyte separation incubated cell separation |
| 1000 | Hematoblast extenterate(thrombin disposal) | |
| 1000 | Lymphocyte washing | |
| SERO rotor for erythrocyte washing | 500 | Blood type determination, the image of the hematocyte |
| 1000 | The test of intersectant aptness | |
| 1000 | Hematocyte washing, the distillation of the serum and plasma |
TD4 cell washer centrifuge it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.
TD4N Table Top 5000rpm Low Speed Laboratory Centrifuge
TD4N Features:
1. Microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, blood center, laboratory and biochemistry.
2. Brushelss motor, free maintenance, no powder pollution, quick in speed up and down.
3. The flexible axle driven system which drive the rotor directly, smooth in operation and small vibration.
4. There are many rotors for your choice, suitable for different specifications meet customers’ different requirements of separation.
5. Micro-computer control system, digital display the RCF, time and speed.
6. Electric lid lock, compact design, super speed and imbalance protection.
TD4N Technical Parameter:
| Max. Speed | 5000rpm | Speed Accuracy | ±20rpm |
| Max. Volume | 6x100ml | Power Supply | AC110V/220V 50HZ/60HZ |
| Max. RCF | 3460xg | Noise | ≤55dBA |
| Timer | 0~99min | Net Weight | 18Kg |
| Dimension | 540x370x280mm | Certifications | CE, ISO & Calibration report are available. |
| Warranty | 1 Year |
Matched Rotors for TD4N:
| Order No. | Rotor Type | Max.Speed(rpm) | Max.Volume(ml) | Max.RCF(xg) |
| 4N-1 | Swing Rotor | 5000 | 6x10ml | 3460 |
| 4N-2 | Angle Rotor | 4000 | 30×7/5ml | 2250 |
| 4N-3 | Angle Rotor | 4000 | 18×15/10ml | 2250 |
| 4N-4 | Angle Rotor | 4000 | 24x10ml | 2200 |
| 4N-5 | Angle Rotor | 4000 | 12×15/7/5ml | 2150 |
| 4N-6 | Angle Rotor | 4000 | 12x20ml | 2200 |
| 4N-7 | Angle Rotor | 5000 | 6x15ml | 2540 |
| 4N-8 | Angle Rotor | 5000 | 12x15ml | 3080 |
| 4N-9 | Angle Rotor | 5000 | 4x50ml | 2520 |
| 4N-10 | Angle Rotor | 5000 | 6x50ml | 2850 |
| 4N-11 | Angle Rotor | 5000 | 4x100ml | 2630 |
| 4N-12 | Angle Rotor | 5000 | 6x100ml | 3130 |
TD4N is a table top centrifuge widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, blood center, laboratory and biochemistry.
