DBCO-PEG4-DBCO is a homobifunctional PEG linker containing two DBCO groups and a hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-DBCO is a homobifunctional PEG linker containing two DBCO groups and a hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:
Cat # 28113 – 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # 28114 – 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)
Figure 1 / 3
Click for expanded view
Reagents supplied – Cat # 28115
Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.
Norgen’s Exosomal RNA Isolation Kit (for the extraction of RNA from EV’s that have been isolated using IZON’s qEV columns) constitutes an all-in-one system for the isolation of exosomal RNA from exosomes previously isolated using IZON’s qEV columns or concentrated using IZON’s EV Concentration kit. This kit allows for the isolation of RNA from intact extracellular vesicles (EVs) where the purification is based on spin column chromatography that employs Norgen’s proprietary resin.
The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PR, reverse transcription PCR, Nothern blotting, RNase protection and primer extension, and expression array assays.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15–25°C). This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Cat. 68910 (50 Preps) |
|---|---|
| Lysis Buffer A | 2 x 30 mL |
| Lysis Additive B | 7 mL |
| Wash Solution A | 38 mL |
| Elution Solution A | 6 mL |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
