DBCO-PEG4-NHS ester

K-RINTDF

SKU: 700004335

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Dietary Fiber
Assay Format:	Enzymatic
Detection Method:	Gravimetric/HPLC
Signal Response:	Increase
Limit of Detection:	0.5 g/100 g
Total Assay Time:	~ 3 h work (over 1-2 days)
Application examples:	Food ingredients, food products and other materials.
Method recognition:	AACC Method 32-60.01, AOAC Method 2022.01, AOAC Method 2017.16, ICC Standard Method No. 185 and CODEX Method Type I

The Rapid Integrated Total Dietary Fiber Assay Kit method is validated under collaborative study (AACC Method 32-60.01, AOAC Method 2022.01, AOAC Method 2017.16, ICC Standard No. 185) and is recognized as a Type I Method by CODEX Alimentarius. The K-RINTDF method is the recommended one for the measurement of total dietary fiber in all foods that may or may not contain resistant starch. This method is updated to be more consistent with in vivo conditions in the human small intestine, i.e. a 4 h incubation time. Under these conditions more accurate measurement of resistant starch is obtained, including phosphate cross-liked starch (RS₄). Use of higher enzyme concentrations ensures that resistant maltodextrins produced from non-resistant starch under the incubation conditions of the Integrated Total Dietary Fiber procedure (AOAC Methods 2009.01 and 2011.25) are no longer produced.

In this improved, rapid method, the incubation time with PAA + AMG is reduced to 4 h and the levels of both PAA and AMG are increased to ensure that resistant starch levels obtained with a set of control samples are consistent with ileostomy data. Under these conditions, the DF values obtained for most samples are the same as those obtained with AOAC Methods 2009.01 and 2011.25.

The dietary fiber fractions that are measured with this method are:

1. High Molecular Weight Dietary Fiber (HMWDF) including Insoluble Dietary Fiber (IDF) and High Molecular Weight Soluble Dietary Fiber (SDFP; soluble dietary fiber which is precipitated in the presence of 78% aqueous ethanol), and

2. Low Molecular Weight Soluble Dietary Fiber (SDFS; water soluble dietary fiber that is soluble in the presence of 78% aqueous ethanol).

Alternatively, IDF, SDFP and SDFS can be measured separately.

The enzymes used in this method are high purity and effectively devoid of contaminating enzymes active on other dietary fiber components such as β-glucan, pectin and arabinoxylan. They are supplied as freeze-dried powders; allowing the use of glycerol as an internal standard in the method.

See our full range of dietary fiber assay kits.

View Dietary Fiber Measurement Guide – Which Method for which sample?

* See McCleary, B. V., Sloane, N & Draga, A. (2015). Determination of total dietary fibre and available carbohydrates: a rapid integrated procedure that simulates in vivo digestion. Starch/Starke, 66, 1-24.

Validation of Methods

Advantages

• More rapid measurement – incubation time with PAA + AMG reduced to 4 h in comparison with AOAC 2009.01 (increased levels of enzyme employed) 
• DF values for most samples are very similar to those obtained with AOAC Method 2009.01 
• Rapid Integrated Total Dietary Fiber method removes all of the limitations that have been identified with AOAC Method 2009.01* 
• All reagents stable for > 2 years after preparation 
• The method is consistent with the CODEX Alimentarius definition of dietary fiber 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Very competitive price (cost per test)

Overview

• Detection kits for the HPV 6/16
• Available in TaqMan format for analysis

More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. HPV 16 has been considered as a high-risk cancer associated HPV type. The low-risk HPV type 6 has been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA.

HPV 6/16 TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

HPV 6/16 TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 5

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	TaqMan Probe Cat. TM42050 (100 preps)	TaqMan Probe/Primer and Control Set Cat. TM42010 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 µL	–
HPV 6/16 Primer & Probe Mix	280 µL	280 µL
HPV 6/16 Positive Control	150 µL	150 µL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

100 bp DNA Ladder in 2% of agarose gel.

• For sizing and quantification of double strand DNA fragments.
• Composed of nine bands as shown on right.
• The 500-bp band with higher concentration is easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.