DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.
DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.
This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column. The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes. The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.
Figure 1 / 2
Click for expanded view
| Kit Specifications | |
| Maximum Blood Input | 3 x 3 mm diameter punches |
| Average yield* (ng) | 150 ng |
| Column Binding Capacity | > 50 µg |
| Time to Complete 10 Purifications | 35 minutes |
* Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 36000 (50 preps) |
|---|---|
| Digestion Buffer B | 6 mL |
| Lysis Buffer B | 20 mL |
| Wash Solution WN | 18 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 1.2 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes | 50 |
| Product Insert | 1 |
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA and miRNA from cell and tissue without MagZol reagent |
| Applications | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Cell, Animal tissue, Plant tissue |
| Sample amount | Cells: ≤ 5 x 10^6, Animal tissue:<10mg |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.
Advantages
Kit Contents
| Contents | R431102 | R431103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Proteinase K | 48 mg | 240 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer RLC | 40 ml | 200 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
TD6C Table top low speed centrifuge
TD6C Features:
1. Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.
2. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.
3.super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
4. Adapters are available by experiment requirements.
5. 3 tiers protection steel cover, safe and reliable.
TD6C Technical Parameters:
| Max speed | 6000rpm | Max RCF | 4110×g |
| Max volume | 6×100ml | Power supply | AC 110V/220V 50HZ |
| Timer | 0～99min | Noise | ≤55dBA |
| Dimension(WxDxH) | 485×320×320mm | Speed accuracy | ±20rpm |
| Net Weight | 18Kg | Certifications | CE, ISO & Calibration report is available |
Matched Rotors for TD6C:
| Order No. | Rotor | Max Speed (rpm) | Max Volume(ml) | Max. RCF（×g） |
| 6C-1 | Angle Rotor | 6000 | 6×15ml/7ml/5ml | 3660 |
| 6C-2 | Angle Rotor | 5000 | 12×15ml/7ml/5ml | 3080 |
| 6C-3 | Angle Rotor | 6000 | 6×50ml | 4110 |
| 6C-4 | Angle Rotor | 6000 | 4×50ml | 3620 |
| 6C-5 | Angle Rotor | 6000 | 4×100ml | 3790 |
| 6C-6 | Angle Rotor | 5000 | 6×100ml | 3130 |
