DBCO-PEG6-amine TFA salt

Introduction

Usages:
For the growth of pathogenic and non-pathogenic microorganisms.

Principle:
Polypeptone provide carbon and nitrogen sources, vitamins and growth factors; Neisseria starch can promote the growth characteristics and enhanced hemolytic streptococci; sodium chloride to maintain osmotic balance; agar as culture medium coagulant.

Formulation (per liter):
Peptone: 10g
Beef extract powder :10g
Sodium chloride: 5g
Agar :15g
Final pH 7.4 ± 0.2

How to use:
1. Suspend 40g of the product, adding 1 L of distilled or deionized water, ,heated to boiling stirring until completely dissolved, dispensing into flask, 121 autoclaved 15min.
2.After the medium is cooled to about 50 , sterile procedures, basal medium was added per 100mL defibrinated sheep or rabbit blood 5-10mL, shake pour plate; also dispensing sterile tubes set into the slope. Spare.
3. take the bacteria to be tested fresh pure cultures inoculated crossed or coated on the plate, set the temperature of the incubator training requirements were cultured for a predetermined time.
4. Observe the results.

Quality control:
The following were inoculated after 36 ± 1 for 24h , the results are as follows:
bacteria name bacteria No. growth status colony characteristics
Escherichia coli ATCC25922 not good hemolysis

Staphylococcus aureus, ATCC6538 good β-hemolytic

Beta-hemolytic streptococcus CMCC (B) 32210 good β-hemolytic

Streptococcus pneumoniae CMCC (B) 31001 good α-hemolytic

Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.

500g

Gel images of different ranges of library size selection. Sheared human genomic DNA was used as input.

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Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.

There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.

Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.

The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.

Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.

NGS library size selection with dual clean-ups.

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Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.

NGS library size selection with single clean-up for >5 kb and >10 kb libraries.

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Features of NGS library size selection

• • • 5 ranges for NGS library size selection
      • • illumina PE100 sequencing: 250-350 bp
        • illumina PE150 sequencing: 300-450 bp
        • illumina PE100 sequencing: 450-750 bp
        • Long-read sequencing: >5 kb
        • Long-read sequencing: >10 kb
    • Rapid protocol: only 20 minutes
    • Simple workflow
      • • No columns required
        • No gel purification required
        • No centrifugation required