Overview

Standard profile polypropylene, universal 96 well non-skirted plate, designed for use on standard thermal cyclers.

• 0.1 ml wells
• Made using Polypropylene
• Cut corner A12
• Working volume: <100 µl, total well capacity: 200 µl
• Universal 96 well non-skirted plate
• Designed for use on standard thermal cyclers

Standard profile polypropylene, universal 96 well non-skirted plate, designed for use on standard thermal cyclers.

Introduction

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	0.2-0.6ml
Elution volume	≥30μl
Time per run	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labour

Kit Contents

Contents	IVD5432
Purification Times	200
MagPure Particles G	14 ml
Carrier RNA	310 μg
Proteinase K	180 mg
Protease Dissolve Buffer	10 ml
Buffer MLK	250 ml
Buffer MAW1	250 ml
Buffer MW2*	2 x 50 ml
Elution Buffer	60 ml

Contents	IVD5432-F-96A	IVD5432-F-96B	IVD5432-F-96C
Sample amount	200～350μl	400~700μl
Carrier RNA	110 μg	110 μg
Proteinase K	50 mg	100 mg
Protease Dissolve Buffer	5 ml	6 ml
Elution Buffer	15 ml	15 ml
Tip	1	1
Sample Plate A	500μl Buffer MLK	500μl Buffer MLK
Sample Plate B	/	500μl Buffer MLK
Wash Plate 1	700μl Buffer MAW1	700μl Buffer MAW1
Wash Plate 2	25μl Buffer MPG2700μl Buffer MW2	25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3	700μl Buffer MW2	700μl Buffer MW2
Elution Plate	/	/

Contents	IVD5432-TL-06A	IVD5432-TL-06B	IVD5432-TL-06C
Sample amount	300~350μl	600~700μl	900~1050μL
Carrier RNA	310 μg	310 μg	310 μg
Proteinase K	50 mg	100 mg	150 mg
Protease Dissolve Buffer	6 ml	6 ml	10 ml
Elution Buffer	15 ml	15 ml	15 ml
DA-Tip	12	12	12
Row 1/7	600μl Buffer MLK	600μl Buffer MLK	600μl Buffer MLK
Row 2/8	/	600μl Buffer MLK	600μl Buffer MLK
Row 3/9	600μl Buffer MAW1	600μl Buffer MAW1	600μl Buffer MLK
Row 4/10	20μl Buffer MPG2600μl Buffer MW2	20μl Buffer MPG2600μl Buffer MW2	30μl Buffer MPG2900μl Buffer MAW1
Row 5/11	600μl Buffer MW2	600μl Buffer MW2	900μl Buffer MW2
Row 6/12	/	/	/

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details

K-CITR

SKU: 700004274

72 assays (manual) / 720 assays (microplate) / 840 assays (auto-analyser)

Content:	72 assays (manual) / 720 assays (microplate) / 840 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Citric Acid
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Decrease
Linear Range:	1.0 to 100 µg of citric acid per assay
Limit of Detection:	0.491 mg/L
Reaction Time (min):	~ 5 min
Application examples:	Grape juice, wine, beer, fruit juices, soft drinks, tea, dairy products (e.g. cheese), meat, processed meat, vegetable and fruit products, bakery products, paper, pharmaceuticals, cosmetics and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by MEBAK, OIV, EU, ISO2963, AOAC and IFU22 (NOTE: If the enzyme oxaloacetate decarboxylase is present in the sample, some of the oxaloacetate product is converted to pyruvate. Therefore, to ensure citric acid is measured quantitatively, D-lactate dehydrogenase (D-LDH) is employed to efficiently convert any pyruvate produced into D-lactate and NAD+).

The Citric Acid test kit is a flexible and simple method for the rapid and reliable measurement and analysis of citric acid (citrate) in foods, beverages and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View our full list of organic acid test kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• Reconstituted citrate lyase stable for 4 weeks at 4^oC / 6 months below -10^oC 
• Buffer / cofactor / enzyme tablets for efficient use of kit components 
• PVP incorporated to prevent tannin inhibition 
• Very competitive price (cost per test) 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included
• Suitable for manual, microplate and auto-analyser formats

The Citric Acid test kit is a flexible and simple method for the rapid and reliable measurement and analysis of citric acid (citrate) in foods, beverages and other materials.