DBCO-PEG8-NHS Ester is a click chemistry molecule cosisting of an NHS ester that is reactive specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG8 spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG8-NHS Ester is a click chemistry molecule cosisting of an NHS ester that is reactive specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG8 spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample
No sample splitting or need to use phenol or precipitation methods
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)
Content:
110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Acetic Acid
Assay Format:
Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
up to 1.8 g/L of acetic acid per assay
Limit of Detection:
~ 10 mg/L
Reaction Time (min):
8 min at 25oC or 5 min at 37oC
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Improved method
The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.
As part of Megazyme’s overall commitment to providing the highest quality products, we have developed this acetic acid kit that provides a specific and rapid assay for use with auto-analysers. The kit assay is based on the conversion of NAD+ to NADH and therefore provides a positive reaction (increase in absorbance) which offers a more robust assay.
The reagents, as supplied, are stable for a minimum of 2 years and the prepared reagents are stable for a minimum of 1 week (on-board stability). In addition, the prepared reagents can be stored frozen for longer term stability (see K-ACETGK Booklet for more details).
View more of our acetic acid and organic acid assay kits.
Advantages
Excellent reagent stability
> 7 days at 4oC or > 2 years below -10oC when prepared for auto-analyser applications
> 2 years as supplied
Very rapid reaction (~ 5 min at 37oC)
Linear calibration (R2 ~ 0.997 up to 1.8 g/L sample)
Document
The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.
