DBCO-PEG8-NHS Ester is a click chemistry molecule cosisting of an NHS ester that is reactive specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG8 spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-PEG8-NHS Ester is a click chemistry molecule cosisting of an NHS ester that is reactive specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG8 spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
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For the detection of the SARS-CoV-2 variants with the 20I/501Y.V1, VOC-21FEB-02 and variants carrying the E484K mutation Rapid detection of specific detection profiles High priming efficiency Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
