DBCO STP ester

DBCO STP ester is a water-soluble reagent with a terminal DBCO group and a STP ester group. DBCO STP ester that can be used for the modification of peptides, antibodies, proteins, and other molecules containing the amine group. The STP esters are excellent alternatives to conventional N-hydroxysuccinimide (NHS) esters for coupling reactions in aqueous environments. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. Reagent grade, for research use only.

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Urine DNA Isolation Kits

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Overview

Rapid isolation of both small and large species of DNA from urine
Convenient spin column format
Effective removal of PCR inhibitors
Purified DNA is highly suited to sensitive downstream applications
Allows for the purification of viral DNA from urine

Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 – 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.

This kit is fully compatible with Norgen’s Urine Collection and Preservation Tubes.

Urine DNA Isolation Kit

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.

Urine DNA Isolation Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.

Urine DNA Isolation Maxi Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.

Background

DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.

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Supporting Data

Figure 1 / 8

Figure 1. A Typical Agarose Gel Showing Total Urinary DNA

Figure 2. Isolation and Detection of DNA from 1.75 mL Urine Samples

Figure 3. Circulating DNA Isolated from Urine

Figure 4. Isolation of Total Urinary DNA from Different Urine Volumes

Figure 5. Isolation and Detection of DNA from Different Urine Volumes

Figure 6. Highly Sensitive Isolation of Viral DNA

Figure 7. Total Urinary DNA Isolated from Different Urine Volumes

Figure 8. Linear Decrease of Ct Values with Increasing Urine Input

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Kit Specifications – Spin Column
Minimum Urine Input	3 mL
Maximum Urine Input	25 mL
Time to Complete 10 Purifications	< 30 minutes
Size of Urine DNA Purified	Large (> 1 kb) and small (150-250 bp)

Kit Specifications – 96-well
Urine Input Volume	3 – 25 mL
Elution Volume	1st Elution 100 μL 2nd Elution 100 μL
Size of DNA Purified	Large (> 1 kb) and small (150-250 bp)
Time to Complete 96 Purifications	< 1 hour

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Slurry B1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry B1 contains a grey resin that will not dissolve when warmed.

Component	Cat. 18100 (50 preps)	Cat. 48800 (50 preps)	Cat. 50100 (50 preps)
Binding Solution K	15 mL	–	–
Slurry B1	–	18 mL	18 mL
Proteinase K in Storage Buffer	2 mL	–	–
Pronase K in Storage Buffer	2 mL	–	–
Soluton WN	9 mL	–	–
Binding Buffer A	–	–	50 mL
Lysis Buffer A	–	30 mL	30 mL
Wash Solution A	–	38 mL	38 mL
Wash Solution B	30 mL	–	–
Wash Solution D	9 mL	–	–
Binding Solution K	15 mL	–	–
Elution Buffer B	15 mL	15 mL	15 mL
Micro Spin Columns	50	–	–
Mini Filter Spin Columns	–	50	50
Collection Tubes	50	50	50
Elution Tubes (1.7 mL)	100	100	100
Product Insert	1	1	1

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Propargyl-PEG24-amine

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TD4N Table Top 5000rpm Low Speed Laboratory Centrifuge

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Max. Speed	5000rpm	Speed Accuracy	±20rpm
Max. Volume	6x100ml	Power Supply	AC110V/220V 50HZ/60HZ
Max. RCF	3460xg	Noise	≤55dBA
Timer	0~99min	Net Weight	18Kg
Dimension	540x370x280mm	Certifications	CE, ISO & Calibration report are available.
Warranty	1 Year

Order No.	Rotor Type	Max.Speed(rpm)	Max.Volume(ml)	Max.RCF(xg)
4N-1	Swing Rotor	5000	6x10ml	3460
4N-2	Angle Rotor	4000	30×7/5ml	2250
4N-3	Angle Rotor	4000	18×15/10ml	2250
4N-4	Angle Rotor	4000	24x10ml	2200
4N-5	Angle Rotor	4000	12×15/7/5ml	2150
4N-6	Angle Rotor	4000	12x20ml	2200
4N-7	Angle Rotor	5000	6x15ml	2540
4N-8	Angle Rotor	5000	12x15ml	3080
4N-9	Angle Rotor	5000	4x50ml	2520
4N-10	Angle Rotor	5000	6x50ml	2850
4N-11	Angle Rotor	5000	4x100ml	2630
4N-12	Angle Rotor	5000	6x100ml	3130

DBCO STP ester

Detail

Other Products

Urine DNA Isolation Kits

Overview

Details

Supporting Data

Propargyl-PEG24-amine

TD4N Table Top 5000rpm Low Speed Laboratory Centrifuge

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