Description

Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.

Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.

Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations.  Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.

Detection of the SARS-CoV-2 variants with the 20B/S.484K mutation, also known as P2
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target Positive copy number standard curve for quantification Accurate controls to confirm findings 96 reactions, includes master mix

Description

Specifications

Clone	IHC697
Source	Mouse Monoclonal
Positive Control	Colon Cancer
Dilution Range	1:200

Thymidylate Synthase (TS) is a crucial enzyme responsible for the synthesis of 2′-deoxythymidine-5′-monophosphate (dTMP) a precursor for thymidylate which is necessary for DNA replication and repair from 2′-deoxyuridine-5′-monophosphate (dUMP). In terms of cancer, TS is an important target for cancer treatment as the inhibition of TS and therefore nucleotide synthesis necessary for cell growth has shown to be a vital part for successful treatment against colorectal, pancreatic and breast cancers.

Introduction

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.

Details

Specifications

Features	Specifications
Main Functions	Co-isolation DNA and RNA from FFPE tissue
Applications	RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method	Polydisperse silicon based magnetic beads (DNA)Monodisperse carbonyl magnetic beads (RNA)
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor and pipetting workstation
Sample type	FFPE slice, FFPE puncture sample, embedded tissue
Sample amount	No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area
Yield	DNA: 1 – 10 μg,  RNA: 1 – 25 μg

Principle

The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.

Advantages

• High quality – high purity total RNA / DNA can be directly used in various downstream applications
• Safe – no phenol chloroform extraction required
• Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
• Post digestion sorting – higher DNA and RNA yields
• High RNA yield – monodisperse carbonyl adsorption

Kit Contents

Contents	R632701	R632702	R632703
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagBind Particles	1.1 ml	2.5 ml	11 ml
MagPure Particles N	1.1 ml	2.5 ml	11 ml
Proteinase K	24 mg	48 mg	220 mg
Protease Dissolve Buffer	3 ml	10 ml	15 ml
Buffer DPS	50 ml	100 ml	2 x 250 ml
Buffer ATL	20 ml	30 ml	120 ml
Buffer BST1	20 ml	40 ml	200 ml
Buffer BXW1*	44 ml	110 ml	3 x 110 ml
RNase Free Water	15 ml	30 ml	120 ml

Storage and Stability

Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.

Experiment Data

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.