DBCO-Sulfo-Link-biotin is an anqueous soluble biotin reagent for introducing a biotin moiety to proteins and cell surfaces containing azide moiety through copper-free Click Chemistry. This biotin reagent is membrane-impermeable due to its negatively charged, water soluble sulfonate group. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-Sulfo-Link-biotin is an anqueous soluble biotin reagent for introducing a biotin moiety to proteins and cell surfaces containing azide moiety through copper-free Click Chemistry. This biotin reagent is membrane-impermeable due to its negatively charged, water soluble sulfonate group. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Blood Urea Nitrogen Enzymatic Kit is a microplate-based colorimetric assay for the determination of urea in serum samples produced from blood. Blood urea nitrogen (BUN) is an important marker for normal kidney and liver function. Elevation of BUN levels is often an indication of intestinal and kidney obstruction and cardiac failure. Decreased BUN levels are often associated with kidney and liver damage. BUN is also a very useful tool for preclinical investigation of experimental drug formulations and BUN levels are commonly used to monitor and attenuate the toxic effects of experimental drug formulations in rodents.
Blood Urea Nitrogen Enzymatic Kit uses an enzyme-based assay to determine urea in liquid samples such as serum. The test is based on a highly proven method for urea determination. The Blood Urea Nitrogen Enzymatic Kit contains sufficient materials to test 42 samples in duplicate. The assay utilizes urease, a metabolic enzyme, to specifically detect urea in serum. The Blood Urea Nitrogen Enzymatic Kit provides rapid, accurate, proven results even in complex liquid mixtures. The limit of detection for the test is 8 ppm urea for serum. The linear range of the assay is 8 – 200 ppm analyte.
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96 determinations
Rapid and simple method
Minimal sample prep
Highly accurate and reproducible
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Details
Specifications
Features
Specifications
Main FunctionsC
Co-isolation DNA and RNA from a single FFPE tissue sample
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
FFPE slice, FFPE embedded tissue
Sample amount
No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area.
Principle
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. For DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA was finally eluted with low-salt buffer.
Advantages
1.Use non-toxic dewaxing solution without contact with xylene 2.Obtain both DNA and RNA simultaneously from the same sample. Elute separately without affecting each other (Have the same steps and effects as top brand 80234, perfect substitute.)
Kit Contents
Contents
IVD5116
Purification Times
50 Preps
HiPure DNA Micro Column
50
HiPure RNA Mini Column I
50
2ml Collection Tubes
150
Proteinase K
50 mg
Protease Dissolve Buffer
5 ml
Buffer DPS
60 ml
Buffer FRL
15 ml
Buffer ATL
15 ml
Buffer RLC
15 ml
Buffer AL
15 ml
Buffer VHB
44 ml
Buffer RW2
25 ml
RNase Free Water
10 ml
Buffer AE
10 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.