DBCO-Sulfo-Link-biotin is an anqueous soluble biotin reagent for introducing a biotin moiety to proteins and cell surfaces containing azide moiety through copper-free Click Chemistry. This biotin reagent is membrane-impermeable due to its negatively charged, water soluble sulfonate group. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-Sulfo-Link-biotin is an anqueous soluble biotin reagent for introducing a biotin moiety to proteins and cell surfaces containing azide moiety through copper-free Click Chemistry. This biotin reagent is membrane-impermeable due to its negatively charged, water soluble sulfonate group. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-t-butyl ester comprises a propargyl group and a t-butyl protected carboxyl group. The propargyl group reacts with azide compounds via copper catalyzed Click Chemistry to form a stable triazole linkage. Under acidic conditions, the carboxyl group can be deprotected. The hydrophilic PEG units enhance solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-t-butyl ester comprises a propargyl group and a t-butyl protected carboxyl group. The propargyl group reacts with azide compounds via copper catalyzed Click Chemistry to form a stable triazole linkage. Under acidic conditions, the carboxyl group can be deprotected. The hydrophilic PEG units enhance solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Character
Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.
Refrigeration Series
● Dual compressors,Secop special compressors,air cooling (water cooling system is optional)
● Refrigeration method:Cascade refrigeration method,high cooling efficiency,good reliability,using CFC-Free environmentally friendly refrigerant.
Silicone oil conducts heat,with uniform temperature and accuracy up to 0.1°C.Silicone oil has a wide temperature range,up to-100°C~+600°C.
● Shelf refrigeration system and cold trap refrigeration system are completely independent,without any switching,refrigeration temperature is lower and more stable.
Vacuum series
● Vacuum pump:German Pfeiffer anti-corrosion,anti-oil return function vacuum pump.(Vacuum pump can be selected according to user needs)
● Pumping rate:9m³/h~62m³/h,multiple specifications are optional,the ultimate vacuum can reach 2×10-3mbar,and the oil mist filter is standard.
● The vacuum pipelines are all high-vacuum stainless steel vacuum tubes, which can effectively prevent vacuum leakage and corrosion.
● The double anti-oil return device completely eliminates the damage and
equipment and samples.
● Automatic backfill air device,the system has a built-in High efficiency particulate air(HEPA)filter to ensure the complete cleanliness of backfill air.
● The vacuum stabilization system can stabilize the vacuum degree of the freeze-drying process through the micro-pass vacuum control valve to ensure that the samples will not be affected by excessive fluctuations in the vacuum degree.
● Condensed liquid collection and discharge system,automatically completes the collection and discharge of condensed liquid after freeze-drying.
Control System Characteristic
● Control:Automatic/Manual
● Can store at least 100 independent freeze-drying programs,each program contains 32 program segments (Can be
customized according to user needs),each program segment corresponds to a processing stage (pre-freezing,main
drying,post-drying).and Include the following segment values:Cycles,shelf temperature,vacuum and safety pressure
● All the equipment can be recorded in real time and the corresponding freeze-drying curves and data tables can be generated after the end.The records of all data and curves comply with the corresponding regulations and standards.
● It has hierarchical management authority,hierarchical management allocation authority and usage process tracking authority
● Remote System
O Fully support 2G/4G/GSM/Wi-Fivarious connection modes
o Users can remotely view all data on the device and operate it
o Technicians can remotely perform maintenance and fault detection on equipment,and can remotely guide and train users
o Can remotely upgrade and change the system of the equipment
Parameter
| Mode | MP2 | MP5 | MP8 | MP12 |
| Freeze-dried area | 0.24m | 0.48m² | 0.96m² | 1.45m² |
| Ice condenser capacity | 4kg | 9kg | 12kg | 18kg |
| Shelf temperature | 70℃~+80℃ | 65℃~+70℃ | ||
| Ice condenser temperature | 70 /-90℃ | 70/-90℃ | -70℃/-90℃ | -70℃/-85℃ |
| Cold trap volume | 9L | 15L | 18L | 30L |
| Chamber type | single | double | ||
| Max number of shelves | 2+1 | 5+1 | 8+1 | 12+1 |
| Shelf size | 275*400 mm | 275*400 mm | 365*465 mm | 365*465 mm |
| Shelf spacing | 100mm(adjustable) | 100mm(adjustable) | 70mm(adjustable) | 70mm(adjustable) |
| Sample quantity (4R Cillin bottle) | 558 | 1395 | 2630 | 4230 |
| Shelf heating and cooling method | Thermal conducting silicone oil medium(temperature resistance:-100℃~+600℃) | |||
| Chamber and shelf surface | Atomization and electrolytic surface treatment,no dead corner residue | |||
| Pipeline diameter | 中 8 9 m m | |||
| External valve port | 6/ | 12/ | 18/ | |
| Equipment power | 3.8kw | 5.5kw | 6.5kw | 8kw |
| Equipment size | 700*588*1600 mm | 1030*700*1630 mm | 1030*700*1850 mm | 1500*850*1900 mm |
| Capping mode | Hydraulic system gland device/no gland | Hydraulic pressure | ||
| Suitable clean room | suitable | |||
| Vacuum sensor type | PiraniVacuum sensor/capacitive vacuum sensor | |||
| Remote control and alarm | Support 2G/4G/GSM/Wi-Fi | |||
| Terminal judgment function | Pressure contrast method Pressure test/pressure contrast method | |||
| Freeze dryer front door cover | Stainless steel/Plexiglass | Stainless steel | ||
| Inert gas filling | (Optional)Sufficient amount and time for the program to run itself | |||
| User function customization | Can be customized and modified according to user needs | |||
| Sterilization method | HzOz/steam sterilization | |||
| Note:”/”optional.Pilot models can be customized according to user needs | ||||
Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.
Magnetic Beads (tRNA Purification)
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
The Magnetic Beads (tRNA Purification) solves the tRNA and short DNA/RNA purification problem. The beads with our proprietary technology purify tRNA effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents can be used for oligo (>70 nt) applications.
Workflow without large RNA/DNA contamination
In the case of samples contaminated with RNA/DNA such as rRNA and DNA, our magnetic beads can effectively remove RNA/DNA that are 180 nt and larger. Purified tRNA are ideal for applications requiring high quality, as the fragments are free of impurities and contaminants.
Workflow with large RNA/DNA contamination
Comparison of tRNA and 70 nt oligos recovery. BioDynami beads (tRNA purification) successfully recovered DNA fragments both yeast tRNA and 70 nt oligos.
Recovery of tRNA and 70 nt oligos with BioDynami magnetic Beads (tRNA Purification). Yeast tRNA and 70 nt oligos were used as input. Input and recovered oligos were quantified with ssDNA Quantification kit (BioDynami Cat. # 40043) and RNA Quantification kit (BioDynami Cat. # 40044).
Depletion of larger RNAs. Left panel: depletion of 28S rRNA and 18S rRNA. Right panel: depletion of RNA of 180 nt and larger.
Features
Removal of unwanted components and other impurities
Purification of tRNA and oligos (>70 nt)
tRNA
RNA fragments 70 nt or longer
DNA/RNA hybrid fragments 70 nt or longer
Oligo and chimeric oligo 70 nt or longer
dsDNA fragments 70 bp or longer
ssDNA fragments 70 nt or longer
Removal of larger RNA/DNA contamination:
18S rRNA
28S rRNA
RNA/DNA> 180 nt
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
