Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate
Applications
PCR, southern bolt and virus detection, etc
Purification method
96 well plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principle
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
High throughput – 96 samples can be processed simultaneously
Kit Contents
Contents
D311701
D311702
Purification Times
1 x 96
4 x 96
HiPure gDNA Plate
1
4
96 well Plate (2.2ml)
1
4
1.6ml Collection Plate
1
4
0.5ml Collection Plate
1
4
Silicon Seal Tape
1
4
Seal Film
5
25
Buffer ATL
30 ml
100 ml
Buffer AL
30 ml
100 ml
Buffer DW1
60 ml
250 ml
Buffer GW2
50 ml
2 x 100 ml
Proteinase K
50 ml
200 ml
Protease Dissolve Buffer
5 ml
15 ml
Buffer AE
30 ml
120 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. Programmable microprocessor control system, touch screen indicates rotor No., speed, time, temperature, RCF, rate of speed as well as fault information.
2. Adopts band heater, fast heating speed, temperature uniform, with the function of temperature-control and constant.
3. Centrifugal tube made in special skill, anti-broken when work.
4. Great toque brushless motor, maintenance-free, no powder, fast acceleration and deceleration, low noise, which improve the stability a lot.
5. Electric lid lock, over-speed and over-temperature protection
6.3 phase to keep the best performance.
7. Stainless steel chamber, safe and reliable
TD5B Technical Parameter:
Max. Speed
4000rpm
Max. RCF
2498×g
Max. Capacity
4×100ml
Time Range
0~99min
Rotor
Swing Out Rotor
Noise (dB)
≤ 58
Temperature
Room temperature:+10~80℃
Acc/Dcc
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Oil tube size
37×200mm
Size (W x D x Hmm)
685×500×385mm
Net Weight(Kg)
67KG
Document
Oil test centrifuge, Heating oil test centrifuge, special tube oil test centrifuge