Dengue virus, Zika virus and Chikungunya virus multiplex kit
Facebook
X
Pinterest
Email
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
The genesig® Dengue, Zika and Chikungunya Virus Multiplex kit is designed for the detection and differentiation of Dengue virus, Zika virus (ZIKV) and Chikungunya virus (CHIKV) only. Individual tests have been designed in the conserved regions of each virus such that all isolates and subtypes will be detected simultaneously in the same test. The Dengue component of the test will detect subtypes 1, 2, 3 and 4 but will not differentiate between them. A positive Dengue test results indicates that the sample has either one of these four subtypes.
The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis. They therefore have a very broad quantification profile.
Other Products
HCM013 Muller-Kauffmann Tetrathionate
Product Info
Document
Product Info
Introduction
Intended Use
For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017.
Principle and Interpretation
Meat extract and casein provide a source of nitrogen and amino acids and sodium chloride maintain theosmotic balance. Ox bile and brilliant green act as selective agents against non-target microorganisms. Tetrathionate is generated from the sodium thiosulfate. Iodine and calcium carbonate buffer the sulfuric acid generated from tetrathionate reduction.
Formulation
Ingredients
/liter
Meat extract
4.3g
Enzymatic digest of casein
8.6g
Sodium chloride
2.6g
Calcium carbonate
38.7g
Sodium Thiosulfate (anhydrous)
30.4g
Ox bile
4.78g
pH8.0±0.2 at 25°C
Preparation
Suspend 89.4g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks,and then cool to below 45°C. Add a vial of novobiocin sodium salt (SR0640), a vial of iodine solution and a vial of brilliant green (SR0040) into 100 mL of base medium. Mix thoroughly.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24 hours
Quality control strains
Approx. Inoculum(CFU)
Expected Results
Salmonella typhimurium ATCC14028
10 – 100
≥ 10 cfu on XLD
Escherichia coli ATCC25922
> 104
≤100 cfu on TSA
Enterococcus faecalis ATCC29212
> 104
<10 cfu on TSA
Storage and Shelf Life
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
References
ISO 6579:2017 Microbiology of food and animal feeding stuffs – Horizontal method for the detection, enumeration and serotyping of Salmonella spp.
Document
Intended Use For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Principle and Interpretation……
This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 x 106 cell using two columns (gDNA removed column)
Applications
RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 mins. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 25 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Kit Contents
Contents
R411102
D411103
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RLC
50 ml
200 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
12 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Document
This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.