Diazo Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. The hydrophilic spacer arm provides better solubility to the labeled molecules in aqueous media. Diazo allows efficient release of captured biotinylated molecules from streptavidin using sodium dithionite (Na2S2O4). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Diazo Biotin-PEG3-alkyne is useful for introducing a biotin moiety to azide-containing biomolecules using Cu(I)-catalyzed Click Chemistry. The hydrophilic spacer arm provides better solubility to the labeled molecules in aqueous media. Diazo allows efficient release of captured biotinylated molecules from streptavidin using sodium dithionite (Na2S2O4). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.
Blood DNA Isolation Midi Kit
Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.
Blood DNA Isolation Maxi Kit
This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.
Blood DNA Isolation 96-Well Kit (High Throughput)
This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.
Blood DNA Isolation Kit (Magnetic Bead System)
Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications | |
| Minimum Blood Input | 20 µL |
| Maximum Blood Input | 200 µL |
| Column Binding Capacity | > 50 µg |
| Average Yield (200 µL of blood) | 4-12 µg* |
| Time to Complete 10 Purifications | 30 minutes |
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 46300 (50 preps) | Cat. 46380 (100 preps) | Cat. 51400 (20 preps) | Cat. 31200 (12 preps) | Cat. 46350 (192 preps) | Cat. 59800 (50 preps) | Cat. 62600 (192 preps) |
|---|---|---|---|---|---|---|---|
| Lysis Buffer B | 20 mL | 2 x 20 mL | 2 x 40 mL | 2 x 110 mL | 2 x 40 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
| Solution WN | 18 mL | 2 x 18 mL | 55 mL | 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 2 x 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 30 mL | 2 x 30 mL | 30 mL | 30 mL | 2 x 30 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
| Proteinase K in Storage Buffer | 1.2 mL | 2 x 1.2 mL | 4.4 mL | 8 mL | 4.5 mL | 1.2 mL | 4 mL |
| Magnetic Bead Suspension | – | – | – | – | – | 2 x 1.1 mL | 8.5 mL |
| Maxi Spin Columns | – | – | – | 12 | – | – | – |
| Mini Spin Columns | 50 | 100 | – | – | – | – | – |
| Midi Spin Columns | – | – | 20 | – | – | – | – |
| 96-Well Plate | – | – | – | – | 2 | – | 2 |
| Adhesive Tape | – | – | – | – | 4 | – | 2 |
| Collection Tubes | 50 | 100 | 20 | 12 | – | – | – |
| 96-Well Collection Plate | – | – | – | – | 2 | – | – |
| Elution Tubes | 50 | 100 | 20 | 12 | – | 50 | – |
| 96-Well Elution Plate | – | – | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Description
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
