[DL2000] ExcelDye™ 6X DNA Loading Dye, Green, 5 ml x 2
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The ExcelDye™ 6× DNA Loading Dye (Green) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 800 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Detail
Description
The ExcelDye™ 6× DNA Loading Dye (Green) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 800 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.03% Xylene cyanol FF
0.15% Orange G
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months -20°C for 36 months
Other Products
Stool Total RNA Purification Kit Dx
Product Info
Document
Product Info
Overview
CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Ideal for use in in vitro diagnostic workflows
Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
Isolate full diversity of RNA from large RNA down to small and microRNAs
Eliminates PCR inhibitors including humic acids
High quality RNA for sensitive downstream applications
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen’s Stool Nucleic Acid Collection and Transport Devices Dx (Cat. Dx45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes.
NOTE: This product is not available for sale in the United States.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
20 bp DNA Ladder on 3.6% of agarose gel, 8% and 12% of acrylamide gel.
• For sizing and quantification of double strand DNA fragments. • Composed of eight bands as shown on right. • Premixed with 6X DNA loading buffer for direct gel loading.
Document
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
Produced by reverse osmosis, ion-exchange, electro-deionization, ultrafiltration, UV treatment (UVC & 185 nm), 0.1 μm membrane filtration
Nuclease-free, protease-free, endotoxin-free
Suitable for molecular biology applications (qPCR, NGS etc.)
Suitable for cell culture applications
Produced in an ISO certified GMP laboratory
Water system is routinely monitored for compliance with QC standards
The Norgen water purification system has been designed in conjunction with experts from Millipore Sigma. The Ultrapure NFW is purified in a two-stage process. The first stage, to produce type II water, includes pre-treatment with activated charcoal and polyphosphate, this water then undergoes reverse osmosis, electro-deionization and ion exchange to remove contaminants as well as UV treatment for disinfection. The Pure water then flows to a second system in a clean room with an additional 4 layers of purification. After passing through an additional ion exchange column, the water is then subjected to Ultrafiltration to remove particulates, bacteria, & ionic contaminants down to trace level, the water is then treated with UV light (182 nm wavelength) to break down organic matter. Lastly a second Ultrafilter for the production of pyrogen-, nuclease- and bacteria-free water at 18.2 MΩ. This water is of utmost purity and suitable for direct use in molecular biology applications such as qPCR and NGS, as well as cell culture and other applications.
Product Specifications
pH
n/a (highly pure water does not contain enough ions for an exact pH determination.)
Recommended Storage
Room Temperature
Grade
Ultrapure (ASTM Type I)
Biological Activity
DNase-, RNase-, Protease-, Endotoxin- Free
TOCs
< 50 ppb
Resistivity
> 18 MΩ-cm
Treatment
Not DEPC-Treated
Quantity
100 mL, 500 mL, 1000 mL
Purification Methods
Activated charcoal, polyphosphate, reverse osmosis, electro-deionization, ion exchange, UV, ultrafiltration
Shipping Conditions
Room Temperature
Details
Applications For use in any molecular biology application
Storage Conditions Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.
Precautions and Disclaimers This product is designed for research purposes only. It is not intended for human or diagnostic use.
