DL6MB large capacity refrigerated centrifuge 

Overview

• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
• Versatile sample input ranges
• Isolate all sizes of free-circulating RNA, including microRNA​
• Bind and elute all RNA irrespective of size or GC content, without bias
• The purified exosomal RNA is free from any circulating RNA-binding proteins
• No phenol extractions, Proteinase K treatment nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes are required
• No precipitation reagents nor overnight incubation required
• Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Details

Supporting Data

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Kit Specifications
Minimum Urine Input	2 mL
Maximum Urine Input	10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50 – 100 µL
Time to Complete 10 Purifications	35 – 40 minutes
Average Yields*	Variable depending on the specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component	Cat. 59200 (50 preps)	Cat. 59300 (25 preps)	Cat. 59400 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	2 x 20 mL	2 x 20 mL	2 x 20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	2 x 18 mL	18 mL	18 mL
Elution Solution A	2 x 6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	100	50	30
Collection Tubes	100	50	30
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

Control  System:   

 5.4”LCD   Touch   Screen   Display

>The display can simultaneously display:all main parameters such as cold trap temperature,vacuum degree,sample temperature, and shelf temperature.

>Schematic diagram of equipment operating components,through graphic display to view the working status of each component of the equipment.

>Ithas  the  functions  of  cold  trap  precooling  and  vacuum  pump  preheating,and  the  time  can  be  set  by  the  userin  the  system. >Display  whether  the  current  running  status  is  normal  or  not  by  numerical  color.

>Built-in    saturated    vapor     pressure    &temperature    corresponding     relationship    table,dearly     displaying    the    corresponding     relationship.

o One-key operation,the operation is extremely simple,no special professional learning and training is required.

o Thermal electromagnetic over-current and short-circuit protection,making the equipment run more safely and stably. 

o Active reminder of equipment maintenance time and vacuum pump replacement lubricating oil time.

o Ambient    temperature    monitoring,the    device    automatically     reminds    the    alarm    if    the    ambient    temperature     is    too    high.

o Optional eutectic point detection device and endpointjudgment detection system make freeze-drying more scientific.

Drying chamber	· The separate drying chamber reduces the probability of damage caused byimproper handling of the drying chamber due to height and weight.The upper cover is anodized aluminum with anti-corrosion treatment.●Highly transparent plexiglass drying chamber,good light and heat radiation conduction.Reserve 6/12 external   valve ports.· 316L stainless steel all black Teflon coating drying chamber,suitable for drying organic solvents and  various corrosive samples,reserved for 6/12 external ports.· 316L stainless steel vertical multi-port multi-manifold external rack(The standard configuration is aplexiglass drying chamber,and one ofthe diying chambers can be optional)
Cold trap andcondensing coil	· The cold trap cavity and condensation coil are made of 316L stainless steel.Large opening and channel design,the gas can be quickly captured through the condensing coil.· The cavity and condensing coil are equipped with Teflon anti corrosion coating as standard,which can trap organic solvents and various corrosive solvents.· The condensing coil is placed in the cold trap to increase the condensate catchment area,which can effectively prevent external condensation.
Sample shelves	· 316Lstainless steel stackable sample shelves,capable oflyophilizing machine solvents and various corrosive  solvents.Each shelf can be placed and taken freely without manual work.The distance and height ofeach shelfcan be adjusted freely.-All black coating,good heat absorption performance.-Special hole for freeze-drying of centrifuge tubes,which is conducive to the drying of samples in centrifugetube containers.· All-aluminum Teflon surface anti-corrosion treatment electric heating shelf.
Refrigerationsystem	· Danffos compressorCopper plate heat exchanger,the largest area to increase heat dissipation· Unique refrigeration technology,the temperature of the cold trap can be lowered from ambient temperatureto below-80C°within 2 minutes.· Class A temperature sensor with high temperature accuracy and low error.· Multiple detection and alarm system,the refrigeration system pressure is too high or refrigeration failure cantimely alarm and take corresponding measures.

Techinical parameter

Item No	ST7003	ST7006		ST9006
Ice condenser capacity	3kg	6kg		6kg
Ice condenser temperature	-70℃	-70℃		-90℃
Cold trap volume	5L	10L		10L
Freeze dried chamber	Bell shape (standard configuration)/Multi manifold type/T-shaped frame type
Sample shelf	φ200mm.Single layer area 0.03m2 with 3-5 layers available	●260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optiona
Vacuum pump	Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types
Oil mist filte	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
Anti-corrosive treatment	Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents.
External valve	6pieces/optional	1-48 pieces/optional
Eutectic point detection system	Support/Optional
Data output analysis	Support/Optional
Powe	0.6kw	1.1kw		1.5kw
Size	420*545*455mm		510*570*500mm

Freezer Dryer, 3kgs-6kgs water capturer
Model:
ST7003,ST7006,ST9006

Brand:
Yingtai

Overview

• Purify amplified DNA ranging from 100 bp -15,000 bp in size
• Fast and efficient spin column format 
• Available in a 50 prep size and a 250 prep size
• Also available in 96 well format
   

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

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Kit Specifications – 96-well
Binding Capacity Per Well	15 μg
Maximum Loading Volume Per Well	500 μL
Size of DNA Purified	100 – 15,000 bp
Average DNA Recovery	> 90%
Average Primer Reoval	> 90%
Minimum Elution Volume	50 μL
Time to Complete 96 Purifications	20 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 14400 (50 preps)	Cat. 45700 (250 preps)	Cat. 24800 (192 preps)
Binding Buffer C	30 mL	5 x 30 mL	3 x 30 mL
Wash Solution A	12 mL	2 x 20 mL	2 x 38 mL
Elution Buffer B	8 mL	2 x 30 mL	2 x 15 mL
Spin Columns	50	250	–
Collection Tubes	50	250	–
96-Well Plate	–	–	2
Adhesive Tape	–	–	4
96-Well Collection Plate	–	–	2
Elution Tubes (1.7 mL)	50	250	–
96-Well Elution Plate	–	–	2
Product Insert	1	1	2