Introduction

A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.

Details

Specifications

Principle

The CleanSeq method contains magnetic particles in an optimized binding buffer to selectively capture sequencing extension products. The protocol can be performed directly in the thermal cycling plate. Unincorporated dyes, nucleotides, salts and contaminants are removed using a simple washing procedure. The purification procedure is amenable to a variety of automation platforms since it requires no centrifugation or vacuum filtration.

Advantages

• High recovery – up to 90%
• High throughput – using magnetic beads purification technology
• Simple – simplified operation process, combination – washing – elution

Kit Contents

Storage and Stability

CleanSeq Beads should be stored at 2-8°C upon arrival and is stable up to 6 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Mix CleanSeq Beads well before using. The reagent should appear homogenous and consistent in color.

DO NOT FREEZE.

A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.

Introduction

Usages:

For pre-enrichment of Salmonella spp.

Principle:

Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; potassium dihydrogen phosphate and disodium hydrogen phosphate as buffer.

Formulation(per liter):
Peptone: 10g
Sodium chloride:5g
Disodium hydrogen phosphate:3.5g
Potassium dihydrogen phosphate: 1.5g
Final pH7.2 ± 0.2

How to use:
1. Suspend 20g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min, set aside.
2.Diluted and treated samples.

Quality control：
          　　

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle; 225ml*10bag/box

Reference:
1.GB/T4789.4-2003 People’s Republic of China national standards of food hygiene Examination of Salmonella microorganisms
2.GB/T4789.28-2003 People’s Republic of China national standards of food hygiene microbiological examination Staining, media and reagents
3.SN0170-92 People’s Republic of China Import and Export Commodity Inspection industry standard Salmonella food for export (including Arizona bacteria) test method
4.GB/T4789.4-2008 People’s Republic of China national standards of food hygiene Examination of Salmonella microorganisms
5.GB 4789.4-2010 national standards for food safety standards of food hygiene inspection and microbiological testing of salmonella People’s Republic of China
6.GB 13091-91 People’s Republic of China National Standard Test Method for Salmonella in feed
7.GB 4789.40-2010 national standards for food safety standards of food hygiene inspection microorganism People’s Republic of China E.sakazakii test
8. ISO 6579-2002 Microbiology of food and animal feeding stuffs —– Horizontal method for the detection of Salmonella spp.
9. ISO22964-2006 Milk and milk products —– Detection of Enterobacter sakazakii
10. ISO6785-2001 Milk and milk products —– Detection of Salmonella spp

250g