[DM2360] FluoroBand™ 100 bp+3K Fluorescent DNA Ladder, 500 μl

Overview

• Versatile plasma/serum input ranges
• No phenol extractions
• Fast and easy processing of samples for the purification and the isolation of RNA.
• No time-consuming ultracentrifugation.
• More effective than any other method.
• EXTRAClean resin separation matrix provides highly purified RNA

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. These kits can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS.

Background

Cell-free circulating RNA, including exosomal RNA in plasma or serum, has the potential to provide biomarkers for certain cancers and disease states, and includes tumor-specific extracellular RNA in the blood. Exosomes are 40 – 100 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which depend upon the tumour cell type from which they are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses, they can be efficiently recovered from biological fluids, such as plasma or serum.

EXTRAClean Plasma/Serum RNA Purification Mini Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 μL to 200 μL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 μL to 25 μL.

EXTRAClean Plasma/Serum RNA Purification Midi Kit

This utilizes a two-column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 μL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.

EXTRAClean Plasma/Serum RNA Purification Maxi Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.

Details

Supporting Data

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Kit Specifications
Sample Type	Plasma/Serum
Anti-Coagulant (for Plasma)†	EDTA or Citrate
Sample Volume Range	250 μL to 1.5mL
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	35 – 40 minutes
Size of RNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Average Yields¥	Variable depending on specimen

† This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR

¥ Please check page 6 for Average Plasma/Serum Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component	Cat. 73700 (50 preps)	Cat. 73710 (20 preps)	Cat. 73720 (10 preps)
Lysis Buffer A	2 x 20 mL	100 mL	1 x 130 mL 1 x 30 mL
Wash Solution A	18 mL*	1 x 38 mL* 1 x 18 mL*	38 mL*
Elution Solution A	6 mL	6 mL	6 mL
Elution Buffer F	–	–	15 mL
EXTRACLean Micro Spin Columns	50	–	–
EXTRACLean Mini Spin Columns	–	20	10
EXTRACLean Midi Spin Columns	–	20	–
EXTRACLean Maxi Spin Columns	–	–	10
Collection Tubes	–	20	10
Elution Tubes (1.7 mL)	50	20	10
Product Insert	1	1	1

Overview

• Fast and easy processing using either a rapid spin column or automation friendly high-throughput 96-well plate format
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• Bind & elute all RNA irrespective of size or GC content, without bias
• Highly sensitive & linear down to a few cells/viral copies without the need for carrier RNA
• No phenol or chloroform extractions
• For use on preserved saliva collected with Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200)
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• CE-IVD marked version available

Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.

Saliva/Swab RNA Purification Kit (Spin Column)

This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.

Saliva/Swab RNA Purification 96-Well Kit (High Throughput)

High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.

Details

Supporting Data

Figure 1 / 8

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Kit Specifications
Sample Volume Range	250 μL
Size of RNA Purified	All sizes, including small RNA(<200 nt)
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	15 – 20 minutes
Average Yield	≥ 1 μg **Varies from sample to sample

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Kit Component	Cat. 69100 (50 preps)	Cat. 69300 (192 preps)
Lysis Buffer A	30 mL	100 mL
Solution WN	18 mL	55 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Solution A	6 mL	20 mL
Mini Spin Columns	50	–
Collection Tubes	50	–
Elution Tubes (1.7 mL)	50	–
96-Well Isolation Plate	–	2
96-Well Collection Plate	–	2
96-Well Elution Plate	–	2
Adhesive Tape	–	4
Product Insert	1	1