[DM2360] FluoroBand™ 100 bp+3K Fluorescent DNA Ladder, 500 μl
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The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Detail
Description
The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 3,000 bp
Concentration
56 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light Room temperature for 6 months 4°C for 12 months -20°C for 24 months
Animal Disease Kit is a diagnostic kit designed for animal detection. It is suitable for the detection of various diseases, including cat and dog test kits, in animals. The kit is composed of various components such as primers, probes, reagents, buffers and other materials necessary for the detection of diseases, which can be used for test strips and other detection methods. The kit can be stored at -20°C for long-term use. With this Animal Disease Kit, you can easily detect diseases in animals and protect them from harm.
The Animal Disease Kit includes multiple components, such as primers, probes, reagents, buffers, etc., to detect diseases. The kit offers various detection methods, including test strips, making it suitable for a broad range of applications in animal disease detection.
Animal Disease Kit is widely used in cattle veterinary medicine, animal disease detection, and animal feed. The kit has been proved to be reliable and efficient in the detection of animal diseases. It helps to diagnose diseases quickly, providing an accurate basis for subsequent treatments.
Support and Services:
We are committed to providing quality products and services to our customers. Our Animal Disease Kit is designed for veterinary professionals to diagnose and treat medical conditions in animals.
1.Technical Support
Our technical support staff are available to answer any questions you may have about the Animal Disease Kit. We provide assistance with product installation, troubleshooting, and any software or hardware issues you may encounter. We also offer technical advice on how to use the kit and its features.
2.Customer Service
Our customer service team is available to help you with any inquiries you may have about the Animal Disease Kit. We assist with product returns, replacement parts, and warranty information. We also provide product information and advice on how to get the most out of your Animal Disease Kit.
FAQ:
Q1: What is the “Animal Disease Kit” product?
A1: The “Animal Disease Kit” is a comprehensive toolkit for the diagnosis of animal diseases.
Q2: What does the Animal Disease Kit include?
A2: The Animal Disease Kit includes Primers, Probes, Reagents, Buffers, Etc.
Q3: How do I use the Animal Disease Kit?
A3: The Animal Disease Kit is designed for easy and convenient use. You can refer to the instructions manual for detailed instructions on how to use the product.
Q4: How accurate is the Animal Disease Kit?
A4: The Animal Disease Kit is designed for accurate and reliable results. It has been tested and certified by qualified professionals for accuracy and reliability.
Q5: Is the Animal Disease Kit safe to use?
A5: Yes, the Animal Disease Kit is safe to use. All components of the kit are made with non-toxic and non-irritating materials, and all reagents are safe to use.
Document
Animal Disease Kit is a diagnostic kit designed for animal detection. It is suitable for the detection of various diseases, including cat and dog test kits, in animals. The kit is composed of various components such as primers, probes, reagents, buffers and other materials necessary for the detection of diseases, which can be used for test strips and other detection methods. The kit can be stored at -20°C for long-term use. With this Animal Disease Kit, you can easily detect diseases in animals and protect them from harm.
Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:
Cat # 28113 – 100 reactions (sufficient for 100 reactions x 20 µL reaction volume) Cat # 28114 – 200 reactions (sufficient for 200 reactions x 20 µL reaction volume) Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)
2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) – Sufficient reagent for 500 x 20 µL reactions
Storage Conditions and Product Stability 2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.
