Introduction

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications	PCR, qPCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount	Solid tissue : 1-10mg, Anticoagulant blood : 200μl
Yield	1 – 15µg
Elution volume	≥20μl
Time per run	30 – 60 minutes
Liquid carrying volume per column	750µl
Binding yield of column	100µg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast – without separation of leukocytes, organic extraction or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Wide applicability- It can handle various liquid samples, animal tissues and cultured cells

Kit Contents

Contents	IVD3018
Purification Times	100
2ml Collection Tubes	2 x 100
Buffer ATL	60 ml
Buffer AL	60 ml
Buffer GW1	44 ml
Buffer GW2	50 ml
Proteinase K	60 mg
Protease Dissolve Buffer	5 ml
Buffer AE	15 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

Articles

• 【Molecular Cancer 】Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming
• 【BIOSENSORS & BIOELECTRONICS】A multiplexed electrochemical quantitative polymerase chain reaction platform for single-base mutation analysis
• 【eLife】Domain fusion TLR2-4 enhances the autophagy-dependent clearance of Staphylococcus aureus in the genetic engineering goat
• 【TALANTA】Unique dual indexing PCR reduces chimeric contamination and improves mutation detection in cell-free DNA of pregnant women
• 【Microbiology Spectrum】Oral Bacteriome and Mycobiome across Stages of Oral Carcinogenesis | Microbiology Spectrum
• 【Biology of Sex Differences】Integrated chromatin accessibility and DNA methylation analysis to reveal the critical epigenetic modification and regulatory mechanism in gonadal differentiation of the sequentially hermaphroditic fish, Monopterus albus
• 【Cell Reports】Translocational attenuation mediated by the PERK-SRP14 axis is a protective mechanism of unfolded protein response

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

K-ACHYD

SKU: 700004260

50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)

Content:	50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 6 months under recommended storage conditions

Analyte:	Acetaldehyde
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	0.5 to 20 µg of acetaldehyde per assay
Limit of Detection:	0.18 mg/L
Reaction Time (min):	~ 4 min
Application examples:	Wine, champagne, beer, liqueurs, brandy, dairy products (e.g. yogurt), bread, fruit juices, soft drinks, cocoa, vegetable and fruit products, coffee, and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by MEBAK

The Acetaldehyde test kit is a simple, reliable and accurate method for the measurement and analysis of acetaldehyde in beverages and foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View our full range of assay kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• No wasted aldehyde dehydrogenase solution (stable suspension supplied) 
• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Simple format 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The Acetaldehyde test kit is a simple, reliable and accurate method for the measurement and analysis of acetaldehyde in beverages and foodstuffs.

K-ARGA

SKU: 700004264

115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser)

Content:	115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	L-Arabinose, D-Galactose
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 μg of L-arabinose or D-galactose per assay
Limit of Detection:	0.58 mg/L (L-arabinose), 0.69 mg/L (D-galactose)
Reaction Time (min):	~ 12 min (L-arabinose), ~ 6 min (D-galactose)
Application examples:	Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan, arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), food additives (e.g. sweeteners), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Novel method

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

See more of our monosaccharide assay kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• Very rapid reaction due to inclusion of galactose mutarotase (patented technology) 
• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Only enzymatic kit available 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.