As specialists for DNA polymerases, we offer our services to you: DNA polymerase activity test (sequencing PAGE or real-time PEx) DNA polymerase purity (SDS-page) DNA polymerase functionality tests (PCR, PEx, LAMPs, etc) DNA polymerase contaminants (DNA, RNA, DNase, RNAse)
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DNA polymerase activity tests and QC
As specialists for DNA polymerases, we offer our services to you:
DNA polymerase activity test (sequencing PAGE or real-time PEx)
DNA polymerase purity (SDS-page)
DNA polymerase functionality tests (PCR, PEx, LAMPs, etc)
DNA polymerase contaminants (DNA, RNA, DNase, RNAse)
20 bp DNA Ladder on 3.6% of agarose gel, 8% and 12% of acrylamide gel.
• For sizing and quantification of double strand DNA fragments. • Composed of eight bands as shown on right. • Premixed with 6X DNA loading buffer for direct gel loading.
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• For sizing and quantification of double strand DNA fragments. • Composed of eight bands as shown on right. • Premixed with 6X DNA loading buffer for direct gel loading.
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
The product assembled with ultrasonic welded technology
Versatile port design facilitates both pouring and aseptic filling techniques
Gamma radiation sterilization
the cell culture surface area
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
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Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.
RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.
– half-life at 95°C of >40 min.
– fast start function due to a hotstart aptamer formulation which prevents unspecific amplification at lower temperatures (<57°C)
– facilitates “zero-step” RT-PCRs directly from RNA templates (without an isothermal reverse transcription step), as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step.
– allows reverse transcription reactions at high temperatures, thus minimizing the problems encountered with strong secondary structures in RNA that melt at elevated temperatures.
– RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes
Please note: Due to the thermostable nature of the enzyme, it is recommended to design very high melting primers and probes (>65°C). See more details in FAQ.
For research use and further manufacturing.
In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
Document
RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.
RevTaq RT-PCR DNA polymerase can be used similarly to Tth DNA polymerase also for reverse transcription, but does not require the addition of Mn2+ for RT-PCR, which simplifies assay setup and possible sample processing.