DNase activity in a convenient and sensitive lateral flow colormetric assay that delivers results in real time. Great for Quality Testing for DNase contamination of materials and supplies
Detail
DNase activity in a convenient and sensitive lateral flow colormetric assay that delivers results in real time. Great for Quality Testing for DNase contamination of materials and supplies.
Attogene’s DNaseAlarm Lateral Flow test is designed for the sensitive and accurate analysis of DNAse activity in liquid samples. DNase Alarm uses a synthetic DNA substrate that attaches to the streptavidin colloidal reporter molecule (gold) using a 5’ biotin. The DNA substrate also contains a FAM molecule that enables it to be captured by the anti-FAM antibody (test line). In the absence of DNases, the DNA oligo tethers gold to the test line giving a visual test line. When DNases are present, the DNA substrate is degraded, and the gold particles can no longer be tethered to the test line thus, signal is lost. Since the cleavage of the DNA Substrate increases over time when DNase activity is present, results can be evaluated kinetically. This assay has applications for quality control testing and analysis of unit activities of DNase and DNase inhibitors. DNase’s can cause havoc in laboratories working with DNA and are important to perform routine testing.
This test can be used to rapidly and efficiently detect DNase’s in both liquid and on solid surfaces and a perfect tool for monitoring manufacturing.
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R4316 HiPure Circulating DNA/RNA Kit
Product Info
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Product Info
Introduction
This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection. HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
Details
Specifications
Features
Specifications
Main Functions
Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma
Applications
qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method
Midi spin column
Purification technology
Silica technology, DNA filtration technology
Process method
Manual (centrifugation or vacuum)
Sample type
serum, plasma, and other cell-free liquid samples
Sample amount
1-5 ml
Elution volume
≥20μl
Time per run
≤100 minutes
Liquid carrying volume per column
4 ml
Binding yield of column
1 mg
Principle
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finally, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.
Advantages
High yield – most optimized process to obtain maximum free RNA and small RNA
High concentration – low elution volume (>20μl) to ensure nucleic acid concentration
High purity – low alcohol combination, completely remove inhibitor and protein pollution
High recovery – silica gel column technology can recover nucleic acid molecules at the level of PG
Large volume – 1-2ml serum and plasma samples can be processed at one time
Kit Contents
Contents
R431602
D431603
Purification Times
50 Preps
250 Preps
HiPure RNA Micro Columns
50
5 x 50
HiPure Viral Midi Columns
50
5 x 50
15 ml Collection Tubes
50
5 x 50
2ml Collection Tubes
50
5 x 50
Buffer CFL
150 ml
2 x 375 ml
Buffer CFP
30 ml
150 ml
Buffer MGW1*
100 ml
2 x 250 ml
Buffer RW2*
2 x 50 ml
5 x 100 ml
RNase Free Water
10 ml
50 ml
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection. HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
High yield and high quality genomic DNA with no RNA or protein contamination
DNA ready for any application including PCR, qPCR, genotyping and more
Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.
Cells and Tissue DNA Isolation Kit (Spin Column)
Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Micro Kit (Micro)
Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.
Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.
8-10 µg (20 mg of animal tissue) 8-12 µg (3 x 106 cells)
Average Purity (OD260/280)
1.8 – 1.9
Time to Complete 10 Purifications
40 minutes hands-on time (Cat. 59100) 50 minutes hands on time (Cat. 62500)
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
Aflatoxin is the most common food toxin that is harmful to human and animal health. The most frequent aflatoxins are B1, B2, G1, and G2, which can affect the body through respiratory, mucosal, or cutaneous routes, causing an excessive inflammatory response. Aflatoxin can infect crops during their growing stages or even after they are harvested. It mainly targets the liver and can impair the effectiveness of immunization in children, increasing the risk of infection. Aflatoxin detection and quantification in food and feed is a critical part of food and feed safety concerns.
Document
Aflatoxin is the most common food toxin that is harmful to human and animal health. The most frequent aflatoxins are B1, B2, G1, and G2, which can affect the body through respiratory, mucosal, or cutaneous routes, causing an excessive inflammatory response. Aflatoxin can infect crops during their growing stages or even after they are harvested. It mainly targets the liver and can impair the effectiveness of immunization in children, increasing the risk of infection. Aflatoxin detection and quantification in food and feed is a critical part of food and feed safety concerns.
This test can be used to rapidly and efficiently detect DNase’s in both liquid and on solid surfaces and a perfect tool for monitoring manufacturing. 
