DOTA-PEG5-C6-DBCO is a PEG linker containing DOTA and DBCO moieties. DBCO is used in Click Chemistry reactions due to its high strain energy. The DOTA group can be ionized and is susceptible for chelating di- and trivalent cations. DOTA can also be used for imaging diagnostic techniques. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DOTA-PEG5-C6-DBCO is a PEG linker containing DOTA and DBCO moieties. DBCO is used in Click Chemistry reactions due to its high strain energy. The DOTA group can be ionized and is susceptible for chelating di- and trivalent cations. DOTA can also be used for imaging diagnostic techniques. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
The ExcelDye™ 6× DNA Loading Dye (Green) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 800 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
Storage
4°C for 12 months
-20°C for 36 months
The ExcelDye™ 6× DNA Loading Dye (Green) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 800 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Pierceable heat sealing foil which has a high solvent resistance. This seal is resealable, suitable for PCR, compound storage and sample shipping.
For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017.
Meat extract and casein provide a source of nitrogen and amino acids and sodium chloride maintain theosmotic balance. Ox bile and brilliant green act as selective agents against non-target microorganisms. Tetrathionate is generated from the sodium thiosulfate. Iodine and calcium carbonate buffer the sulfuric acid generated from tetrathionate reduction.
| Ingredients | /liter |
| Meat extract | 4.3g |
| Enzymatic digest of casein | 8.6g |
| Sodium chloride | 2.6g |
| Calcium carbonate | 38.7g |
| Sodium Thiosulfate (anhydrous) | 30.4g |
| Ox bile | 4.78g |
| pH8.0±0.2 at 25°C | |
Suspend 89.4g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks，and then cool to below 45°C. Add a vial of novobiocin sodium salt (SR0640), a vial of iodine solution and a vial of brilliant green (SR0040) into 100 mL of base medium. Mix thoroughly.
Cultural characteristics observed after incubation at 35-37°C for 24 hours
| Quality control strains | Approx. Inoculum(CFU) | Expected Results |
| Salmonella typhimurium ATCC14028 | 10 – 100 | ≥ 10 cfu on XLD |
| Escherichia coli ATCC25922 | > 104 | ≤100 cfu on TSA |
| Enterococcus faecalis ATCC29212 | > 104 | <10 cfu on TSA |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
On June 14, 2024
ISO 6579:2017 Microbiology of food and animal feeding stuffs – Horizontal method for the detection, enumeration and serotyping of Salmonella spp.
Intended Use For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Principle and Interpretation……
