Magnetic Beads (Short Oligo Purification)

Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.

Oligo purification can also be performed using spin column-based technology. The oligo size limitation for recovery is around 20 nt, as oligos under 20 nt have a very low recovery rate.

We have developed Magnetic Beads (Short Oligo Purification) Kit for short oligo purification. Our proprietary bead technology enables the recovery of oligos as short as 6 nt. 80% of the 6 nt oligos and 90% of the > 8 nt oligos can be recovered. The reagent also effectively removes impurities and unwanted components such as salts, proteins, dNTPs, detergents, and other contaminants. The magnetic bead reagents are RNase free, and can be used for both DNA and RNA applications.

Recovery rates of of short oligos. Oligos with different sizes were used as input. BioDynami Short Oligo Quantification Kit (Cat. # 40046) was used to quantify the recovery rates with modifications.

Features

• Effective purification of short oligos
  • • 6 nt oligos: 80% recovery rate
    • >8 nt oligos: >90% recovery rate
• Removal of impurities and unwanted reaction components

Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.

Description 

The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system. 

Features

• High sensitivity and signal intensity
• Smart blue contrast dye as a visual aid for reaction setup
• Better compatibility for reverse transcription
• Low background 

Storage 

Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months

The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.

A handheld 8-tube screw cap tube capper/decapper. Securely seal or unscrew an 8-cap row in just 10 seconds, or an entire rack of 96 tubes in 2 minutes.

About

8-Channel Handheld Capper/Decapper – Saving you Time and Effort

The 8-Channel Handheld Capper/Decapper  is a versatile tool designed to increase throughput and save you time and effort. Capable of consistently capping and de-capping multiple tubes simultaneously, this innovative device ensures smooth and efficient lab operations. Its lightweight, ergonomic design reduces operator strain and seamlessly transitions from manual to automated systems, making it an indispensable addition to any laboratory.

Speed and Efficiency

With the ability to securely seal an 8-cap row in just 10 seconds or an entire rack of 96 tubes in 2 minutes, the 8-Channel Handheld Capper/Decapper significantly enhances manual processing throughput. The device guarantees consistent sealing across each tube. Its automatic column-type cap removal and capping performance streamline workflows, ensuring quick and reliable operation. This not only saves valuable time but also reduces the risk of repetitive strain injuries for lab personnel.

Ergonomic and Portable

Designed with user comfort in mind, the 8-Channel Handheld Capper/Decapper is lightweight and ergonomically crafted for effortless screw cap processing. It’s neat, compact design and low-force, dual-button cap ejection system makes it ideal for various laboratory environments. Its included storage stand ensures secure and convenient storage and when not in use.

Convenient Charging and Compatibility

The tool features magnetic contact charging, offering long battery life and fast charging times. Ready to use whenever you are, it efficiently caps, de-caps, or recaps tubes by column or row. Interchangeable cap driver cassettes allow for easy processing of different screw cap tube types and SBS rack formats.

Request a Demo or Speak to an Expert

Experience the efficiency and convenience of the 8-Channel Handheld Capper/Decapper in your lab. Visit our website to request a demo or speak to one of our experts to learn more about how this innovative tool can enhance your laboratory’s operations.

A handheld 8-tube screw cap tube capper/decapper. Securely seal or unscrew an 8-cap row in just 10 seconds, or an entire rack of 96 tubes in 2 minutes.