Isolate genomic DNA from anticoagulated and untreated blood
Rapid and convenient spin column procedure
Isolate DNA from inputs as low as 20 µL
This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column. The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes. The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.
* Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Component
Cat. 36000 (50 preps)
Digestion Buffer B
6 mL
Lysis Buffer B
20 mL
Wash Solution WN
18 mL
Wash Solution A
18 mL
Elution Buffer B
15 mL
Proteinase K
1.2 mL
Micro Spin Columns
50
Collection Tubes
50
Elution Tubes
50
Product Insert
1
Other Products
L-Malic Acid Assay Kit (MegaQuant™ Format)
Product Info
Document
Product Info
K-LMALMQ
60 assays per kit
Content:
60 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
L-Malic Acid
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
505
Signal Response:
Increase
Linear Range:
0.15 to 15 µg of L-malic acid per assay (i.e. 0.007-0.75 g/L with a 20 µL sample volume)
Limit of Detection:
15.4 mg/L
Reaction Time (min):
~ 6 min
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
The L-Malic Acid test kit is suitable for the measurement and analysis of L-malic acid in grapes, grape juice and wine using the MegaQuant™ colorimeter (measurement at 505 nm). Suitable for white and red wines at all stages of the winemaking process.
Highly stable reagents (at least three seasons use)
Very competitive price (cost per test)
Spectrophotometer / laboratory / expertise not required
Very simple procedure
Rapid reaction time (~ 6 min)
Standard included
Document
The L-Malic Acid test kit is suitable for the measurement and analysis of L-malic acid in grapes, grape juice and wine using the MegaQuant™ colorimeter (measurement at 505 nm). Suitable for white and red wines at all stages of the winemaking process.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 1 year under recommended storage conditions
Analyte:
L-Lactic Acid
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.3 to 30 µg of L-lactic acid per assay
Limit of Detection:
0.21 mg/L
Reaction Time (min):
~ 10 min
Application examples:
Wine, beer, soft drinks, milk, dairy products (e.g. cream, milk / whey powder, cheese, condensed milk and yogurt), foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), egg, egg products (e.g. egg powder), baking additives, vinegar, fruit and vegetables, processed fruit and vegetables (e.g. tomatoes), meat products, food additives, feed, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by DIN, GOST, IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK
The L-Lactic Acid (L-Lactate) Assay Kit is used for the specific measurement and analysis of L-lactic acid (L-lactate) in beverages, meat, dairy and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The L-Lactic Acid (L-Lactate) Assay Kit is used for the specific measurement and analysis of L-lactic acid (L-lactate) in beverages, meat, dairy and food products.
DirectBlood Genotyping PCR Kit is a novelty on the market. It allows genotyping in real-time and without any previous DNA isolation. You simply save time and money: directly use EDTA blood without any extraction steps. The DirectBlood Genotyping PCR Kit is optimized to function with a wide range of different SNPs to detect possible nucleotide variations. It has been successfully tested with a variety of hydrolysis probes and hybridization probes.The setup of a genotyping experiment can not be simpler anymore: Mix primers&probes with the Genotying mastermix, add the respective blood sample and start real-time PCR, this is it.
It is even more simple: store the DirectBlood Genotyping PCR Kit at room-temperature. A cool chain is not needed anymore. The included master mix is freeze-dried and dissolves within a few seconds after addition of the rehydration buffer.Additionally, we have already established SNP-assays for you. In case, you can not find your respective SNPs, please provide us with the rs-number of the SNP-assays your are interested-in and you will receive an individual quotation from us.For research use and further manufacturing.In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
DirectBlood Genotyping Kit (#5000) performance with a hydrolysis probe assay
The DirectBlood Genotyping Kit is compatible with a wide range of assays: Here we are detecting a SNP in the Factor V gene (rs6025; G1691A), which is connected with a higher risk for venous thrombosis, with a fluorescent hydrolysis probe assay.
Homozygous wild type blood samples will show a strong signal and amplification plot (blue curve) in channel 2 (Cy5) and none or a very low signal
(red curve) in the other channel 1 (ROX).
Heterozygous blood samples will give an intermediate signal and amplification plots with very similar intensities in both channels 1 and 2 (blue and red curve). Homozygous mutant blood samples, channel 1 (ROX) will show a strong signal and amplification plot (red curve) and channel 2 (Cy5) none or a very low signal and amplification plot (blue curve). So the signals are reversed on homozygous mutant samples.
DirectBlood Genotyping Kit performance with a hybridization probe assay
Here we are detecting the same SNP in the Factor V gene (rs6025; G1691A), which is connected with a higher risk for venous thrombosis, with a fluorescent hybridization probe assay.
Three blood samples with known genotype were used. All the genotypes can clearly be identified. The blue curve shows the specific wild type (homozygous G;G) peak at 61°C. The red curve shows the specific mutant (homozygous A;A) peak at 53°C. A heterozygous case (A;G) will result in both melting curve peaks, respectively at the same specific temperature for wild type and mutant (orange curve).
Document
DirectBlood Genotyping PCR Kit is a novelty on the market. It allows genotyping in real-time and without any previous DNA isolation. You simply save time and money: directly use EDTA blood without any extraction steps.
The DirectBlood Genotyping PCR Kit is optimized to function with a wide range of different SNPs to detect possible nucleotide variations. It has been successfully tested with a variety of hydrolysis probes and hybridization probes.
