[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl
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The FluoroStain™ DNA Fluorescent Staining Dye is designed to be a safer replacement for conventional Ethidium bromide (EtBr) which poses a significant health and safety hazard for its users. The FluoroStain™ DNA Fluorescent Staining Dye offers at least 10 times sensitivity in DNA detection levels, and is capable of detecting double stranded DNA (dsDNA) fragments up to 0.04 ng in electrophoresis analysis. The FluoroStain™ DNA Fluorescent Staining Dye shows a high specificity to the dsDNA, with negligible background signal, making the destaining process entirely optional. FluoroStain™ DNA Fluorescent Staining Dye is compatible with both the conventional ultra violet gel-illuminating systems as well as the less harmful long wave length blue light illumination systems. The emission when bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.
Detail
Description
The FluoroStain™ DNA Fluorescent Staining Dye is designed to be a safer replacement for conventional Ethidium bromide (EtBr) which poses a significant health and safety hazard for its users. The FluoroStain™ DNA Fluorescent Staining Dye offers at least 10 times sensitivity in DNA detection levels, and is capable of detecting double stranded DNA (dsDNA) fragments up to 0.04 ng in electrophoresis analysis. The FluoroStain™ DNA Fluorescent Staining Dye shows a high specificity to the dsDNA, with negligible background signal, making the destaining process entirely optional. FluoroStain™ DNA Fluorescent Staining Dye is compatible with both the conventional ultra violet gel-illuminating systems as well as the less harmful long wave length blue light illumination systems. The emission when bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.
Features:
Excellent for post staining
Sensitivity: 0.04 ng DNA
A safer alternative to EtBr
Compatibility: suitable to blue or UV light
Increased cloning efficiency (blue light)
Storage
Protected from light 4°C for 12 months -20°C for 24 months
Other Products
Microsporidia – IFT
Product Info
Document
Product Info
Name of Product
Microsporidia – IFT
Catalog Number
AF 8100
Short Info
Indirect immunofluorescence assay for the specific diagnostic of human intestinal microsporidiosis.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
Indirect immunofluorescence test
Range/Assay Sensivity
Test Principle
Monoclonal antibodies bind specifically to samples with spores (E. bienusi and E. intestinalis) attached to the slide wells. Unfixed antibodies will be washed away. The presence of spore-specific antibodies is detected with a fluorescent anti-mouse IgG conjugate.
Document
2 x 50 tests Indirect immunofluorescence assay for the specific diagnostic of human intestinal microsporidiosis.
To accommodate the need for high quality enzymes for isothermal amplification ArcticZymes developed the IsoPol® series of polymerases. For isothermal amplification at lower temperatures (37°C and below) we offer two enzymes, IsoPol® DNA Polymerase and IsoPol® SD+. Both enzymes exhibit excellent processivity and high strand displacement activity, having 5’-3’ polymerase activity while lacking both 3’-5’and 5’-3’ exonuclease activity.
IsoPol® SD+ is an engineered version of IsoPol® DNA Polymerase with even stronger strand displacement and higher salt tolerance.
Figures
Properties
Quality Control
ArcticZymes is dedicated to the quality of our products. IsoPol® polymerases are is manufactured at our ISO 13485 certified facility in Norway.
Document
To accommodate the need for high quality enzymes for isothermal amplification ArcticZymes developed the IsoPol® series of polymerases. For isothermal amplification at lower temperatures (37°C and below) we offer two enzymes, IsoPol® DNA Polymerase and IsoPol® SD+. Both enzymes exhibit excellent processivity and high strand displacement activity, having 5’-3’ polymerase activity while lacking both 3’-5’and 5’-3’ exonuclease activity.
For the rapid detection and enumeration of coliform bacteria.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine flora β- galactosidase-glucosidase specific reaction, hydrolysis of the substrate, the release of the color groups produce green colonies on the light yellow plate.
Formulation (per liter):
Peptone :10g
Yeast extract powder: 3g
sodium chloride:5g
sodium lauryl sulfate:0.1g
Agar :12g
Chromogenic substrate 2.7g
Final pH 7.0 ± 0.2
How to use:
1. Weigh 32.8g of this product, adding 1L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing into flask without autoclaving.
2,Take 25g or 25mL of sample with aseptic procedures, added to the flask containing 225mL of sterile phosphate buffered saline (or saline) is shaken thoroughly homogenized with a homogenizer or a 1:10 dilution 1min, then 1:10 dilution continue to select the appropriate serial dilutions of three, two plates each dilution was inoculated.
3, the use of pour method: The medium is cooled in a water bath to about 50 , sterilized petri dish having a diameter 90mm, 1ml samples were inoculated per dish, then poured into about 15ml of the above dissolution medium, and mix to solidify, upside down, 37 for 24 hours.
4, using surface inoculation: cooled to about 50 , shake devoted to the medium in sterile petri dish. Can be stored in the refrigerator for a day or stored at room temperature for several days (dark, 4 ). The sample was streaked method or filter method, 37 for 24 hours.
5, observe the results.
Quality control:
This product appear light yellow after pouring into plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.
Growth of bacteria were cultured bacteria numbers feature
Escherichia coli ATCC25922 good green colonies
Citrobacter ATCC8090 good green colonies
Salmonella typhimurium CMCC50115 good colorless colonies
Enterococcus faecalis ATCC29212 suppressed —–
Storage: Store at 10-30 , dark, cool and dry place, tighten the cap immediately after use. Storage period of two years.
