Echinococcus granulosus – IgG ELISA

Introduction

029010 Gram Stain

Usage:For Gram Stain Test.

Specification: 10ml*4 vials

10ml*4vials

DD5 Low Speed 5000rpm Swing rotor 4x800ml lab Centrifuge Adapters for 5/10/15/50ml tubes.

DD5  Low Speed Large Capacity Centrifuge Application:

DD5 Lab centrifuge widely used in the fields of pharmaceutical factory and laboratory, can hold swing out rotors and angle rotors both.

DD5 Low Speed Large Capacity Centrifuge Features:

1. Brushless frequency motor which in great torque, free maintenance, no powder pollution, quick in speed up and down.

2. Digital display which indicates the program, speed, time, RCF and temperature.

3. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration/deceleration for your choice.

4. There are  kinds of rotors for your choice.

5. Electric lid lock, compact design, super speed and imbalance protection.

6. The centrifuge body is made of high-quality steel, safe and reliable.

DD5 Low Speed Laboratory Centrifuge Technical Parameters:

DD5 Low Speed Laboratory Centrifuge Matched Rotors:

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K-MBG4

SKU: 700004319

100 assays (manual) / 400 assays (auto-analyser)

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(3¹-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

Discover more assay kits for enzyme activity measurement.

Data calculators are located in the Documents tab.

Advantages

• Very cost effective 
• All reagents stable for > 2 years 
• Specific for endo-1,3:1,4-β-glucanase/lichenase 
• Simple, convenient, rapid assay 
• Well suited to automation 
• Malt flour standard and lichenase standard included

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.