This ELISA kit is intended for the quantitative detection of IgG antibodies against Em-18 antigen of Echinococcus multilocularis in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Detail
Name of Product
Echinococcus multilocularis (Em18) – IgG ELISA
Catalog Number
AF 9310
Short Info
This ELISA kit is intended for the quantitative detection of IgG antibodies against Em-18 antigen of Echinococcus multilocularis in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Echinococcus multilocularis Em18 antigen sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
Other Products
XTL-65 Series Zoom Stereo Microscope
Product Info
Document
Product Info
Specification
6.5-65 x continuous zoom stereo microscope
XTL-6565A
XTL-6565B
XTL-6565D
XTL-6565C
Zoom Body
Binocular head,45°decline,360°rotate
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Zoom Body
Trinocular head,45°decline,360°rotate
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Eyepiece
WF10X22mm
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Objective
zoom objective 0.65X—6.5X
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Zoom Rate
1:10
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Total Amplific- ation Rate
the standard configuration is 6.5X-65X
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Auxiliary Lens
0.3X,0.5X,0.7X,1X,1.5X,2X For Option
O
O
O
O
WorkingDistance
110mm
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Djopte
55mm-75mm
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Focus arm
76mm Diameter,32mm pole size
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plate
Black/White plate,Glass plate for option
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Dptiona
Stand
O
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Stand
BL3 base,column support upper and lower light source 3 WLED
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Stand
811 base column support large flat base has no light source
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
Details
Specifications
Features
Specifications
Main Functions
Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate
Soil/Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Advantages
Fast – several samples can be extracted in 40 minutes (after digestion)
High purity – the purified DNA can be directly used in various downstream applications
Good repeatability – silica technology can obtain ideal results every time
High recovery – DNA can be recovered at the level of PG
Kit Contents
Contents
D314401
D314402
D314403
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
HiPure DNA Plate
1
4
20
96 Well Plate (2.2ml)
1
4
20
1.6ml Collection Plate
1
4
20
0.8ml Collection Plate
1
4
20
2ml Bead Tubes
100
400
2000
Buffer SOL
100 ml
360 ml
2 x 900 ml
Buffer SDS
10 ml
36 ml
180 ml
Reagent DX
1 ml
1.8 ml
9 ml
Buffer PS
20 ml
80 ml
400 ml
Absorber Solution
20 ml
80 ml
400 ml
Buffer GDP
150 ml
500 ml
3 x 900 ml
Buffer GW2*
50 ml
100 ml
4 x 200 ml
Buffer AE
30 ml
120 ml
500 ml
Storage and Stability
Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
Sequentially isolate and purify total RNA and DNA from a single sample
Two column system: one for DNA and one for RNA
The RNA column is for the purification of total RNA including microRNA
No need to split the lysate, or to use phenol or precipitation methods
Rapid and efficient spin column procedure – it takes only 30 minutes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a rapid method for the isolation and purification of total RNA and DNA sequentially from a single sample of cultured animal cells and tissues, blood, bacteria, yeast, or fungi. The lysate is passed over two columns: 1) a DNA column and 2) an RNA column. Total RNA of all sizes is purified, including microRNA. Both DNA and RNA are of the highest purity and yield.
These kits are ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA Purification Kits are especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as they eliminate the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications
RNA/DNA Purification Kit (Spin Column)
Maximum column binding capacity of 50 μg for RNA and 20 μg for DNA.
RNA/DNA Purification Micro Kit (Micro)
The purified RNA and DNA fractions can be eluted in as little as 20 μL. Ideal for cell number inputs of 500,000 and as little as 5 cells. Maximum column binding capacity of 35 μg for RNA and 10 μg for DNA.
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues
5 x 106 cells 25 mg (for most tissues)** 200 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg
Time to Complete 10 Purifications
30 minutes
Average Yield*: HEK 293 Cells (1 x 106 cells) HEK 293 Cells (1 x 106 cells) Liver (15 mg) Liver (15 mg)
10-15 μg RNA 2-4 μg DNA 30-35 μg RNA 4-6 μg DNA
*Average Yield will vary depending upon a number of factors including species, growth conditions used, and development stage.
**Tissue inputs of up to 40 mg may be used, however for inputs greater than the recommended 25 mg, cross-contamination of the RNA and DNA fractions is possible.
Storage Conditions and Product Stability Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
