endo-BCN-OH is a click chemistry handle which readily reacts with azides. The primary alcohol allows it to be ligated to other linkers.
endo-BCN-OH is a click chemistry handle which readily reacts with azides. The primary alcohol allows it to be ligated to other linkers.
endo-BCN-OH is a click chemistry handle which readily reacts with azides. The primary alcohol allows it to be ligated to other linkers.
Mycoplasma is the smallest and simplest prokaryotic organism. There is a risk of mycoplasma contamination during cell culture, and mycoplasma contamination of cells has become a common problem worldwide.
Mycoplasma contamination may seriously affect the state of cells, change the gene expression and metabolic characteristics of cells, lead to slow cell growth, abnormal differentiation and death, and seriously affect cell function.
Bacteria, yeast or mold contamination in cell culture can be seen under an optical microscope, but mycoplasma contamination is usually not visible under an optical microscope and must be detected by specific detection methods.
Common methods for detecting mycoplasma contamination include mycoplasma isolation and culture, special biochemical tests such as ELISA and luminescence, and DNA fluorescent staining detection. Among the above detection methods, most of the operation steps are relatively cumbersome, the sensitivity is not high, the mycoplasma types cannot be distinguished, special instruments are required, or the time required is long. The qPCR method is relatively simple and convenient to operate, and the results can be obtained in 2 hours.
• Provide results in less than 2 hours.
Good negative and positive controls.
• No live mycoplasma required: The MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require the use of live mycoplasma for validation
• Can be used with gDNA, inactivated mycoplasma, or live mycoplasma
• Can be used as an alternative to the standard 28-day culture test
• Can be used in conjunction with culture-based methods to provide preliminary results while awaiting the 28-day test results
The MycoSEQ Plus Mycoplasma Detection Kit is part of an integrated workflow for adventitious drug, impurity, and contaminant detection during biopharmaceutical manufacturing. The entire workflow includes the sample preparation kit, which provides a manual sample preparation method, which together with the qPCR analysis can provide results in 2 hours. (Please note that complex matrices may require additional upfront processing steps as described in the various available protocols.)
• Mycoplasma qPCR MIX, store at -15°C to -25°C, store at 2°C to 8°C after first use, protected from light.
• Mycoplasma Positive Control, -15°C to -25°C.
• Mycoplasma Negative Control, store at -15°C to -25°C, store at 2°C to 8°C after first use.
Note: Price not include shipment & duty, contact us to get full quote.
DuckyBio’s Mycoplasma PCR Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for detecting mycoplasma contamination in biological materials such as cultured cells. Designed and tested using criteria often applied toward rapid mycoplasma detection in biotherapeutic manufacturing cell culture lot release, the MycoSEQ Plus kit enables results that meet or exceed guidance on sensitivity and specificity expectations as described in European Pharmacopoeia (E.P. 2.6.7, 2007), US Pharmacopoeia (US63) and Japanese Pharmacopoeia. When used with a suitable sample preparation method, the MycoSEQ Plus kit can detect less than 5 CFU/mL.
Xenotropic murine leukemia virus-related virus (XMRV) is a gammaretrovirus. The virus was first described in 2006 and has since been isolated from human biological samples. XMRV belongs to the family Retroviridae and the genus gammaretrovirus. It has a single-stranded RNA genome that replicates through a DNA intermediate. The virus gets its name due to its close relationship with the murine leukemia viruses (MuLVs). The viral genome is approximately 8100 nucleotides in length and is 95% identical with several endogenous retroviruses of mice. While gammaretroviruses have well-characterized oncogenic effects in animals, they have not been shown to cause human cancers. However, XMRV was recently discovered in human prostate cancers and is the first gammaretrovirus known to infect humans. In addition to prostate cancer, a possible association with chronic fatigue syndrome has been reported, however it has yet to be established whether XMRV is a cause of this disease.
The causal role of XMRV in cancer has yet to be established and the virus does not appear to be capable of transforming cells directly. In prostate cancer, XMRV protein has been found in tumour-associated but nonmalignant stromal cells, but not in the actual prostate cancer cells. This raises the possibility that the virus may support tumorigenesis. In other studies, XMRV proteins and nucleic acids were found in malignant cells.
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM34750 (100 preps) | Cat. TM34710 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| XMRV Primer & Probe Mix | 280 μL | 280 μL |
| XMRV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Figure 1 / 9
Click for expanded view
| Sample Type | Cell-Free Cell Culture Media |
| Sample Volume Range | 20 ml to 35 mL |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 40-45 minutes |
| Average Yields | Variable depending on specimen |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.