TD5B Large Capacity Low Speed Centrifuge

Features:

1. Brushless motor with simpler construction, more reliable performance, longer life and quietly running.
2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.
4. Microprocessor control, TD5B with digital display which indicate the speed, time, RCF in operation, speed raising and reducing quick, operate simply.
5.10 kinds of accelerating and decelerating speed for your choice.

TD5B Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×800ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	685×500×385mm
Net Weight(Kg)	67KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for TD5B

Order No	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(×g)
5B-1	Swing Rotor	4000	4×800ml	3450
5B-2	Swing Rotor	4000	4×750ml	3530
5B-3	Swing Rotor	4000	4×500ml	3380
5B-4	Swing Rotor	4000	4×4×96(well)	2940
5B-5	Microplate rotor	4000	2×4×96(well)	3210
5B-6			2×3×48(well)	2300
5B-7	Swing rotor	5000	4×1×50ml	4730
			4×1×100ml	4730
		4000	4×2×100ml	3020
		4000	4×2×50ml	3200
		4000	4×4×15ml	3200
		4000	4×6×15ml	3200
5B-8	Swing rotor	4000	4×8×15ml	3200
		4000	4×12×5ml vacuum tube	2480
		4000	4×12×7ml vacuum tube	2760
		4000	4×12×10ml vacuum tube	2880
		4000	4×8×10/7/5ml vacuum tube	2790
		4000	4×6×10/7/5ml vacuum tube	2790
		4000	4×4×10/7/5ml vacuum tube	2790
5B-9	Swing rotor	4000	4×24×5ml vacuum tube	2480
		4000	4×31×5ml Khan tubes	2480
5B-10	Swing rotor	4000	4×24×7ml vacuum tube	3140
		4000	4×31×7ml Khan tubes	3140
		4000	4×30×7ml vacuum tube	3140
		4000	4×30×5ml vacuum tube	3140
		4000	4×40×7ml vacuum tube	3270
		4000	4×18×10ml vacuum tube	3140
5B-11	Swing rotor	4000	Dairy bottle	3830
5B-12	Fixed rotor	5000	6×15ml	2540
5B-13		5000	12×15ml	3080
5B-14		5000	24×15ml	3500
5B-15		5000	30×15ml	3830
5B-16		5000	4×50ml	2520
5B-17		5000	6×50ml	2850
5B-18		5000	12×50ml	3860
5B-19		4000	24×50ml	2970
5B-20		5000	4×100ml	2630
5B-21		5000	6×100ml	3130
5B-22		4000	12×100ml	2970

Adapter for 800ml and 500ml bucket

VolumeCup	5ml	7ml	10ml	15ml	50ml
800ml（Round Cup）	23pcs/cup	23	19	14	5
500ml（Round Cup）	18pcs/cup	18	14	9	3
500ml（Square Cup）	20pcs/cup	20	16	12	5

TD5B large capacity centrifuge max capacity can be reached to 4x800ml, can with different adapters for small tubes, 50ml, 15ml, 10ml, 7ml, 5ml, 2ml and so on.

Introduction

This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.

Details

Specifications

Features	Specifications
Main Functions	Isolation gDNA from biological sample and remove host DNA
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Culture medium, swab, parasitic blood, tissue, sputum, etc.
Sample amount	Blood sample, homogenate, plasma, brain effusion, swab immersion solution, centrifuged concentrated liquid, etc.:0.5-1ml
Elution volume	≥50μl
Time per run	≤60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica Column purification. This product efficiently depletes human and animal host DNA and yields enriched bacterial DNA. An optimized combination of mechanical and chemical lysis allows efficient disruption of bacterial cells. Target DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High concentration – the host DNA is digested by dnase, without contamination of host DNA
• High recovery – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time
• Wide applicability – it can be used in blood, tissue, intestinal contents and other samples

Kit Contents

Contents	D314802	D314803
Purification Times	50 Preps	250 Preps
Hipure DNA Mini Columns I	50	2 x 125
2ml Collection Tubes	50	2 x 125
2ml beads Tubes	50	250
Buffer DRB	15 ml	60 ml
Buffer ES	6 ml	30 ml
Reagent DX	0.5 ml	1 ml
Buffer DL	30 ml	120 ml
Buffer GW1	22 ml	110 ml
Buffer GW2	12 ml	50 ml
DNase I (Powder)	6 mg	30 mg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	15 ml	60 ml

Storage and Stability

DNase I and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.

N-(Propargyl-PEG2)-DBCO-PEG3-acid facilitates the creation of triazole linkages with azide-containing compounds using Click Chemistry. The carboxylic group can react with amine-containing entities using coupling agents like HATU for further conjugation.

N-(Propargyl-PEG2)-DBCO-PEG3-acid facilitates the creation of triazole linkages with azide-containing compounds using Click Chemistry. The carboxylic group can react with amine-containing entities using coupling agents like HATU for further conjugation.