Product Details

Kit Size:

48 Reactions

Product Name:

DNA Isothermal Rapid Amplification Kit Fluorescent Type

Kit Type:

DNA

Reaction Volume:

50 μL

Storage Temperature:

-20℃

Application:

Nucleic Acid Amplification

Type:

Fluorescent Type

Reaction Time:

20mins

High Light:

20mins home test kit

, 

DNA home test kit

, 

48 Reactions home dna test kit

Payment & Shipping Terms

Minimum Order Quantity

48T

Price

3.8$/T

Packaging Details

16T/bag,48T/box

Delivery Time

6days

Payment Terms

T/T,Paypal

Supply Ability

100000T/Month

Product Description

DNA Isothermal Rapid Amplification Kit(Fluorescent Type)Guide Manual

Product parameters:

Reagent component ( WLE8202KIT ,16T/bags,48T/Box )
Component	Specification	Quantity	Function
A buffer	1.6ml	1 Tube	Buffer system mainly for stabilizing protein/enzyme and performance
B buffer	0.15ml	1 Tube	Mainly activated systems such as magnesium ions
Positive control template	0.1ml	1 Tube	Mainly the positive plasmid template is used to test the effectiveness of the kit
Positive control primer mix	0.06ml	1 Tube	Mainly the primer combination of the positive control template
Reagent Guide Manua	16T/bags,48T/Box	3 bags	Reagent technology of protein/enzyme system: freeze-dried powder, freeze-dried microspheres

Principle overview

This kit is based on a room temperature and constant temperature nucleic acid rapid amplification technology: at room temperature and constant temperature, the recombinase and primer form the protein/single-stranded nucleotide complex Rec/ssDNA, with the help of auxiliary proteins and single-stranded binding protein SSB , invade the double-stranded DNA template; form a D-loop region at the invasion site, and start scanning the DNA double-strands; after finding the target region complementary to the primer, the Rec/ssDNA complex disintegrates, and the polymerase also binds to The 3′ end of the primer initiates chain extension. This kit relies on the action of exonuclease at 39 ºC, adding specific molecular probes designed based on the template, and using fluorescence monitoring equipment to achieve real-time monitoring of the amplification process of the target fragment. 

Primer design

It is recommended to use primers with a length of 30-35 bp. Primers that are too short will affect amplification speed and detection sensitivity; primers are designed to avoid the formation of secondary structures that affect amplification; the amplicon length is recommended to be 150-300 bp, usually no more than 500 bp. 

Fluorescent probe design

The probe sequence does not overlap with the specific primer recognition site, is 46-52 nt in length, and the sequence avoids palindromic sequences, internal secondary structures, and continuous repeated bases. The probe has four modification sites: the middle position ≥ 35 nt from the 5′ end is labeled with a dSpacer (tetrahydrofuran, THF) as the recognition site for exonuclease; the upstream of the THF site is labeled with a fluorescent group, and the downstream Label a quenching group, the distance between the two groups is 2-4 nt; THF is ≥15 nt from the 3′ end, and the 3′ end is labeled with a modifying group, such as an amine group, a phosphate group or a C3-Spacer. 

Product features and advantages:

This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 20 minutes), and the reaction groups are in dry powder state, which is easy to operate and easy to store.

It can be applied to various brands of fluorescence quantitative PCR instruments, constant temperature fluorescence amplification instruments and other fluorescence detection equipment.

This kit is based on a room temperature and constant temperature nucleic acid rapid amplification technology: at room temperature and constant temperature, the recombinase and primer form the protein/single-stranded nucleotide complex Rec/ssDNA, with the help of auxiliary proteins and single-stranded binding protein SSB , invade the double-stranded DNA template; form a D-loop region at the invasion site, and start scanning the DNA double-strands; after finding the target region complementary to the primer, the Rec/ssDNA complex disintegrates, and the polymerase also binds to The 3′ end of the primer initiates chain extension. This kit relies on the action of exonuclease at 39 ºC, adding specific molecular probes designed based on the template, and using fluorescence monitoring equipment to achieve real-time monitoring of the amplification process of the target fragment.

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissues, blood stain, urine, seminal stain and various forensic samples
Sample amount	Blood:1-100μl, Tissue:<10mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

• Fast – several samples can be extracted in 20 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• High recovery – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time

Kit Contents

Contents	D312502	D312503
Purification Times	50 Preps	250 Preps
Buffer ATL	15 ml	60 ml
Buffer AL	15 ml	60 ml
Buffer GW1*	22 ml	66 ml
Buffer GW2*	20 ml	2 x 50 ml
Carrier RNA	310 μg	2 x 310 µg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml
HiPure DNA Mini Columns I	50	2 x 125
2 ml Collection Tubes	100	5 x 100

Storage and Stability

Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

Experiment Data

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.

GL21MC high speed refrigerated centrifuge

GL21MC high speed refrigerated centrifuge Application:

Widely used in the area of animal and plant molecular biology, cell biology, clinical applications, bio-engineering, mass preparation and blood, protein precipitation, Separating the biology, viruses, sub-cell, cell and the chemicals in the cell.

Features:

1.Brushless frequency motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.

2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.
3.Import compressors fluorine free, double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control..
4. Microprocessor control system, the 10 inches LCD display indicates all the parameters in running,
5. There are more than 20 kinds of rotors for your choice, especial the continuous rotors which help you purify a large number of test solution continuously.
6. There are 30 kinds of program and 10 kinds of accelerating and decelerating speed for your choice.
7.ω2t centrifugation is allowed, which make you have the ideal centrifugation result.
8. 20 kinds of rotors for your choice, especial the continuous rotor help you purify a large amount of test solution continuously.
9. Automatically electric lid lock, super speed, over temperature protection and imbalance protection.
10. The centrifuge body is made of high quality steel, safe and reliable.
11. 3 tiers protection steel cover.

Technical parameters:

Max speed	21000r/min	Max RCF	46140*g
Time range	1~99H59min	Speed accuracy	±50r/min
capacity	6*500ml	Noise	≦65dBA
Weight	235KG	Dimension	865*728*1240mm
Packaged weight	285KG	Packaged dimension	1000*850*1510mm
Temperature range	-20~40℃	Temperature accuracy	±1℃
Power	AC110/220V, 50-60HZ,35A	Rotor identification	Automatic identification
Package	wooden box

Matched Rotors for GL21MC:

Order No.	Rotor	Max speed (r/min)	Max capacity (ml)	Max RCF (*g)
30108	Fixed  rotor	21000	12*10ml	44440
30117	Fixed  rotor	20000	16*10ml	46140
30111	Fixed  rotor	20000	6*50ml	44700
30118	Fixed  rotor	20000	8*30/20ml	42040
30119	Fixed  rotor	15000	8*50ml	27540
30112	Fixed  rotor	15000	6*70ml	25480
30120	Fixed  rotor	12000	8*100ml	19610
30121	Fixed  rotor	11000	4*300ml	18120
30122	Fixed  rotor	10000	6*300ml	15730
30123	Fixed  rotor	10000	6*500ml	18340
30128	Continuous centrifugal rotor	17000	1000ml	31390
30129	Continuous centrifugal rotor	14000	1800ml	21710
30130	Continuous centrifugal rotor	8000	3000ml	9450
30131	Continuous centrifugal rotor	8000	1800ml	8600
30132	Interval rotor	17000	1000ml	31390
30133	Interval rotor	12000	1200ml	19350
30134	Interval rotor	10000	3000ml	14890
30135	Vertical rotor	20000	16*5ml	38340
	Vertical rotor	18000	8*30ml	32650
30180	Swing rotor	15000	4*5ml	21670
30181	Swing rotor	13000	4*30ml	23580
30182	Swing rotor	10000	4*50ml	16240
30694	Bucket rotor	4000	4*4*96holes	2840
30696	Swing rotor	4000	4*500ml	3380

GL21M max. capacity can hold 800ml bottles 4pcs, max speed over 20000rpm
Model:
GL21M

Brand:
Yingtai

Code:
8421199090