endo-BCN-PEG2-NHS ester is aclick chemistry crosslinker containing an NHS ester group. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group is reactive with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
endo-BCN-PEG2-NHS ester is aclick chemistry crosslinker containing an NHS ester group. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group is reactive with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Attogene Fluorescent UniversalLateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a fluorescent lateral flow dipstick assay for detection of DNA and RNA products.
Fluorescent lateral flow assays can be 2-100 fold more sensitive that gold based lateral flow tests.
Formats (fluorescent broad range UV light excitation range of 100nm to 400nm, 655nm emission) Streptavidin conjugate pad):
• Detectable using a black light such as a black light UV flashlight or fluorescent lateral flow reader.
• Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification.
• Test line: anti-FITC/FAM
• Control Line: Biotin
• Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FAM and Dig labelled primers during amplification.:
• Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.
Inquire about custom configurations: sales@attogene.com.
For example, this product can be configured with alternative/custom streptavidin fluorescent particles.
Kit Components
50 -4.5mm Fluorescent Lateral Flow Dipsticks
10 mL Sample assay running buffer
Features & Benefits
Can be used for development of a lateral flow assay for detection of a variety of different molecules such as amplified DNA products from PCR, LAMP and RPA reactions.
No need to stripe capture antibodies
No expensive equipment required (Black Light)
Cost-effective way to screen for further downstream lateral flow assay development.
Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
Kit contents
Champion™ Competent Cells
pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
Champion™ Transformation Protocol Card
Shipping condition
Throughout the shipping process, the temperature is maintained under -70°C.
Storage and expiration
Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.
Document
Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.