endo-BCN-PEG2-PFP ester

Product Description

Amp-future DNA Isothermal Amplification Kit NFO: Freeze-Dried Reagent

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

K-FRGLQR

SKU: 700004283

1100 assays (microplate) / 1100 assays (auto-analyser)

Content:	1100 assays (microplate) / 1100 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 6 months under recommended storage conditions

Analyte:	D-Fructose, D-Glucose
Assay Format:	Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	0.4 to 20 µg of D-glucose plus D-fructose per assay
Limit of Detection:	133 mg/L
Reaction Time (min):	~ 13 min
Application examples:	Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

This product has been discontinued, you may be interested in the following replacement product K-FGLQR; 700007621)

The D-Fructose/D-Glucose (Liquid Ready) test kit is a rapid, reliable and accurate method for the specific measurement and analysis of D-fructose and D-glucose in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for auto-analyser and microplate formats.

Find more of our monosaccharide and oligosaccharide assay kits.

Advantages

• PVP incorporated to prevent tannin inhibition 
• “Ready to use” liquid stable formulation 
• Very competitive price (cost per test) 
• All reagents stable for > 2 years 
• Very rapid reaction (~ 13 min) 
• Standard included 
• Suitable for microplate and auto-analyser formats

This product has been discontinued, you may be interested in the following replacement product K-FGLQR; 700007621)

The Permagen 384-well post magnet plate was designed for use in manual or automation applications for Low Elution high throughput PCR 

Magnetic beads are pulled midway up onto well wall to help eliminate accidental bead pellet disruption and to achieve lower volumes

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, compatible with any magnetic beads, and integrated cushion base to help aid in robot/ consumable inconsistencies

SKU #

MSP384LE

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• High throughput applications 
• Magnets sit lower on wells allowing lower volumes
• Made in USA 
• For Research use only

Compatibility

• Full- skirt 384 well PCR plates
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum  – 39 µL 

Minimum  – 5 µL

The Permagen 384-well post magnet plate was designed for use in manual or automation applications for Low Elution high throughput PCR