endo-BCN-PEG4-t-butyl ester

Overview

Non-woven gas permeable seal that limits evaporation. The seal is peelable, pierceable; suitable for cell culture and seed & insect storage too.

• Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
• Our Gas PermASeal Heat Seal is made from a non-woven material and is designed for use in cell culture, due to its porous nature
• The seal is compatible with polypropylene and polystyrene
• It can be removed by peeling, or it can be pierced with a pipette tip manually, using a liquid handling robot.
• Gas PermASeal Heat Seal can be utilized for effective overnight incubations, during which it demonstrates significant reductions in evaporation compared to lids
• It can also be used for insect and seed storage as it enables gas exchange, whilst providing an inert surface with no adhesive to interfere with the well contents
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Non-woven gas permeable seal that limits evaporation. The seal is peelable, pierceable; suitable for cell culture and seed & insect storage too.

Opentrons OT-2 Tips, 300µL. Optimized for volumes 5µL to 300µL Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.

Opentrons OT-2 Tips, 300µL. Optimized for volumes 5µL to 300µL Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.

Introduction

Intended Use

For selective enrichment culture of Listeria monocytogenes in food.

Principle and Interpretation

Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; esculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.

Formulation

Ingredients	/liter
Enzymatic digest of animal tissues　( Proteose peptone)	5.0g
Enzymatic digest of casein　( Tryptone)	5.0g
Meat extract	5.0g
Yeast extract	5.0g
Sodium chloride	20.0g
Disodium hydrogen phosphate dihydrate	12.0g
Potassium dihydrogen phosphate	1.35g
Aesculin	1.0g
Lithium chloride	3.0g
pH7.2±0.2 at 25°C

Preparation

Suspend 57.4g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃,aseptically add the contents of 1 vial of Fraser Selective Supplement (SR0120) to each 225 mL of base to prepare FB1 solution; Add one reagent (SR0130) to each100 mL of FB2 culture medium to prepare FB2 enrichment solution.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 46~50 hours

Quality control strains	Growth	Characteristics
Listeria monocytogenes ATCC19115 + Escherichia coli ATCC25922 + Enterococcus faecalis ATCC29212	>20 cfu  On PALCAM	Gray to black colony count with black halo
Escherichia coli ATCC25922	< 100 colonies on TSA	Inhibition
Enterococcus faecalis ATCC29212

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

ISO 11290

Intended Use For selective enrichment culture of Listeria monocytogenes in food. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen ……