DBCO-PEG24-TFP ester is a heterobifunctional PEG linker with an azide-reactive DBCO and an amine-reactive TFP ester that is less susceptible to hydrolysis than an NHS ester. The PEG linker improves the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG24-TFP ester is a heterobifunctional PEG linker with an azide-reactive DBCO and an amine-reactive TFP ester that is less susceptible to hydrolysis than an NHS ester. The PEG linker improves the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.

Details

Kit Contents

Contents	D312402	D312403
Purification Times	50	250
HiPure DNA Mini Columns I	50	250
2ml Collection Tubes	50	250
Buffer BGL	40 ml	180 ml
Buffer GXP	30 ml	150 ml
Buffer GW1*	13 ml	53 ml
Buffer GW2*	20 ml	2 x 50 ml
DTT Powder	235 mg	2 x 235 mg
Proteinase K	48 mg	240 mg
Protease Dissolve Buffer	5 ml	15 ml
Elution Buffer	15 ml	60 ml

Storage and Stability

This product can be stored at room temperature (15~25°C) for 18 months. Proteinase K/DTT dry powder can be transported and stored at room temperature. For long-term storage (>6 months), it is recommended to store at -20~8°C. Dissolved Proteinase K should be stored at -20~8°C. The dissolved DTT should be stored at -20°C.

This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.

Overview

• Extract high quality & quantity total RNA including miRNA
• No phenol step required; isolate all RNA in one fraction
• Bind & elute all RNA irrespective of size or GC content, without bias
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Isolate from a wide variety of specimens
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Available in a variety of formats to suit your needs
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A₂₆₀/A₂₈₀ suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 – 25 µL	50 µL – 1 mL	250 µL – 1 mL	5 – 10 mL
35300	Total RNA Purification Micro Kit	20 – 50 µL	1 – 4 mL	2 – 10 mL	10 – 20 mL
17200	Total RNA Purification Kit	50 – 100 µL	4 – 10 mL	11 – 30 mL	20 – 35 mL

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Maximum Amount of Starting Material
Animal Cells	1 x 106 cells
Animal Tissues	10 mg
Blood	100 μL
Plasma/Serum	150 μL
Bacteria	1 x 109 cells
Yeast	1 x 108 cells
Fungi	40 mg
Plant Tissues	40 mg
Time to Complete 96 Purifications	30 minutes
Average Yields*
HeLa Cells (1 x 106 cells)	15μg
E. coli Cells (1 x 109 cells)	50 μg

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17200 (50 preps)	Cat. 37500 (100 preps)	Cat. 17250 (250 preps)	Cat. 17270 (500 preps)	Cat. 24300 (192 preps)	Cat. 24370 (576 preps)	Cat. 24350 (192 preps)	Cat. 24380 (576 preps)
Buffer RL	40 mL	2 x 40 mL	175 mL	350 mL	2 x 40 mL	350 mL	2 x 40 mL	350 mL
Wash Solution A	38 mL	2 x 38 mL	148 mL	1 x 148 mL 1 x 74 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL
Elution Solution A	6 mL	2 x 6 mL	30 mL	60 mL	2 x 20 mL	60 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	100	250	500	–	–	–	–
96-Well Isolation Plate	–	–	–	–	2	6	–	–
96-Well Isolation Plate (Deep Well)	–	–	–	–	–	–	2	6
Adhesive Tape	–	–	–	–	4	12	4	12
Collection Tubes	50	100	250	500	–	–
96-Well Collection Plate	–	–	–	–	2	6	–	–
96-Well Collection Plate (Deep Well)	–	–	–	–	–	–	2	6
Elution Tubes (1.7 mL)	50	100	250	500	–	–
96-Well Elution Plate	–	–	–	–	2	6	–	–
96-Well Elution Plate (Deep Well)	–	–	–	–	–	–	2	6
Product Insert	1	1	1	1	1	1	1	1