endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG8-acid is a BCN-containing crosslinker reagent. The terminal carboxylic acid is reactive with primary amine groups in the presence of activators (e.g. EDC, or HATU). The BCN group enable copper free click chemistry with azide -tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.
Application
Storage Buffer
40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol
Storage
-20°C for 24 months
The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.
Folic acid, is one of the B vitamins used to treat anemia caused by folic acid deficiency.
This kit is a product based on indirect-competitive ELISA, which is fast, easy, accurate and sensitive compared with common instrumental analysis.
Detection limit if folic acid in liquid samples
Highly Sensitive, rapid, robust screening kit for Folic Acid
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample) |
| Applications | SDS-PAGE electrophoresis and western blot, etc |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples |
| Sample amount | Cultured cells: < 10^7Animal tissue: ≤ 20 mgPlant samples: ≤ 150 mgYeast cells: 2 x 10^6 – 5 x 10^7 |
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
Advantages
Kit Contents
| Contents | R521102 | R521103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer GW1* | 22 ml | 66 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 50 ml | 3 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
| Elution Buffer | 10 ml | 30 ml |
| Buffer ALO(5%SDS) | 10 ml | 30 ml |
Storage and Stability
HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
