endo-BCN-PEG8-acid

Description 

The PM5200 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5200 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)

Contents 

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control

Under suggested conditions, PM5200 ExcelBand™ 3-color Pre-Stained Protein Ladder Broad Range resolves 15 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for long term storage 

The PM5200 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5200 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

PRP centrifuge/Platelet rich plasma centrifuge

PRP Application:

Aesthetic & Plastic,Orthopedic & Pain-treatment, Dentistry, Ophthalmology,Veterinary and etc..Our company has been cooperated with Korean and Chinese PRP kit manufacturer to making the PRP centrifuge to our customers.Detail information, please send email to us, sales@cn-centrifuge.com

Features:

1.Widely used in hospital, modern beauty salon and micro plastic hospitals.

DC inverter motor with simpler construction, more reliable performance, longer life and quietly running

2.Smooth in operation, low noise and small vibration.

3.Micro computer control system, digital display the RCF, time and speed. 4.Automatic electric lid, compact design, super speed and imbalance protection.

5.The centrifuge body is made of high-quality steel, safe and reliable.

TD4N Technical Parameters:

Max speed	4000rpm	Max RCF	2600xg
Max volume	4x20ml	Noise	≤55dBA
Timer	0～99min	Net weight	28KG
Dimension(HxDxW)	528×370×280mm	Power supply	AC 220V 50HZ 2A
Speed accuracy	±20rpm	Package	Carton box

PRP Centrifuge widly used for Aesthetic & Plastic,Orthopedic & Pain-treatment, Dentistry, Ophthalmology,Veterinary and etc..Our company has been cooperated with Korean and Chinese PRP kit manufacturer to making the PRP centrifuge to our customers

OverView

HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human  total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results  indicate that HL-dsDNase has minimal impact on RNA quality and quantity.

HL-dsDNase is an engineered version of dsDNase that is rapidly and completely inactivated by incubation for 5 minutes at 58°C with 1 mM DTT at pH 8.0 or above. Chemical inactivation in downstream compatible RT or PCR buffers allows milder heat inactivation and, in some cases, skipping heat inactivation altogether. This makes HL-dsDNase very useful for removal of DNA from RNA preparations since the enzyme may be inactivated using various strategies with reduced risk of auto-degradation of  RNA in the presence of magnesium, making HL-dsDNase an ideal enzyme when working with small volumens of RNA.

HL-dsDNase is also an excellent choice for removal of unwanted external DNA from samples prior to analysis of nucleic acids protected by biological membranes, e.g., bacteria, viruses, and sperm. Especially if using downstream analysis methods that might be affected by host cell DNA, as metagenomic sequencing and STR-profiling. The easy inactivation of HL-dsDNase makes it a fast and efficient alternative to methods as differential extraction, where sample material is often lost.

Recommended applications:

• Removal of genomic DNA from RNA preparations
• Improved removal of host-cell DNA from sperm cells prior to STR-profiling

Key advantages with HL-dsDNase:

• Double-strand DNA specific endonuclease
• Easily heat-inactivated by very moderate heat treatment
• High specific activity
• Useful for removal of DNA from RNA preps

Properties

HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human  total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results  indicate that HL-dsDNase has minimal impact on RNA quality and quantity.