endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.
endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.
endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.
Working volume of 0.36ml
Round well with ‘V’ bottom for maximum liquid extraction
High uniformity from well to well
Raised well rims for reliable closing with heat sealing.
Easy and reliable stacking
Good centrifugation stability up to 6,000 × g for faster protocols and improved sample quality
Manufactured under DNase/ RNase free environment without slip agents, plasticizers or biocides – Materials which could have a negative effect on bioassays
Pure, virgin polypropylene guarantees good extractible performance, high resistance to chemicals, good mechanical stress and working with temperature extremes
Autoclavable (121 °C, 20 min)
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Specifications
| Features | Specifications |
| Main Functions | Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Serum, plasma |
| Sample amount | 0.6ml |
| Elution volume | |
| Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA
| Contents | 1292750 | 12927200 |
| Purification Times | 50 preps | 200 preps |
| MagPure Particles F | 1.2 ml | 4.5 ml |
| MagBind Particles | 1.2 ml | 4.5 ml |
| Proteinase K | 24 mg | 90 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer MLB | 30 ml | 120 ml |
| Buffer GDP | 15 ml | 60 ml |
| Buffer MAW1 | 40 ml | 250 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 60 ml |
Storage and Stability
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
SKU: CB0215
Permagen’s Thermal Combo Block 1.5 mL, 0.2 mL PCR was designed as a temperature control device that can be used manually or on a liquid handling robot