The Primerdesign™ genesig® Kit for Enterocytozoon bieneusi (E. bieneusi) genomes is designed for the in vitro quantification of E. bieneusi genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the E. bieneusi genome. The primers and probe sequences in this kit have 100% homology with a broad range of E. bieneusi sequences based on a comprehensive bioinformatics analysis.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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D2122 HiPure Clean Up 96 Kit
Product Info
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Product Info
Introduction
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate
Applications
PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method
96 well Bind Plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
PCR product, enzymatic reaction
Sample amount
Appropriate
Recovery
≥80%
Elution volume
≥75μl
Time per run
≤60 minutes
Liquid carrying volume per column
800µl
Binding yield of column
20µg
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
High recovery efficiency – ≥80% DNA recovery
Fast – isolation can be completed in 60 minutes
Kit Contents
Contents
D212201
D212202
D212203
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
Buffer GDP
120 ml
400 ml
4 x 400 ml
Buffer DW2
20 ml
100 ml
3 x 100 ml
Elution Buffer
20 ml
20 ml
30 ml
HiPure DNA Plate
1
4
20
2.2ml Collection Plate
1
4
20
1.6 ml Collection Plate
1
4
20
0.8 ml Collection Plate
1
4
20
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Document
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Propargyl-PEG1-acid is a crosslinker with a propargyl group and a carboxylic acid group. The carboxylic acid reacts with primary amine under the activation of HATU or EDC. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG1-acid is a crosslinker with a propargyl group and a carboxylic acid group. The carboxylic acid reacts with primary amine under the activation of HATU or EDC. The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-PABA is an analog of DBCO-Acid with PEG linker and a 4-Aminobenzoic acid (PABA) group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. Reagent grade, for research use only.
Document
DBCO-PEG4-PABA is an analog of DBCO-Acid with PEG linker and a 4-Aminobenzoic acid (PABA) group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. Reagent grade, for research use only.
