Overview

• Rapid and simple procedure to generate digested peptides
• Simultaneous digestion, purification and concentration at once
• Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry
• Peptides are ready for applications such as mass spectrometry and SDS-PAGE
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format.  Trypsin is added to the protein sample and bound to the column.  Salts are washed away and the trypsin is then activated to digest proteins.  Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry.  Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation).   The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more.

Details

Supporting Data

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Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Permagen’s MSPU650R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot and consumable labware inconsistencies for precision pipetting  

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

MSPU650R

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Integrated corner brackets to help keep microplate in place
• Cushion base allowing tips to come in contact with well bottoms
• Made in USA 
• For Research use only

Compatibility

• Round bottom plates 
• Pyramid bottom plates 
• Flat Bottom plates
• Deep well plates
• PCR Plates (Full-skirt)
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Minimum – 5 µL (PCR Plate)

Maximum – 2 mL (Deep Well Plate)

Permagen’s MSPU650R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot and consumable labware inconsistencies for precision pipetting

Introduction

This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

Details

Specifications

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Fast – several samples can be extracted in 120 minutes by column method
• Safe – no phenol chloroform extraction required

Kit Contents

Storage and Stability

Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K and MagBind Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.