Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
IL-6 / Interleukin-6 Test
Product Info
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Product Info
Name of Product
IL-6 / Interleukin-6 Test
Catalog Number
MQL6 1
Short Info
Test for the semi-quantitative evaluation of human Interleukin-6 (IL-6) in serum, plasma, cell culture supernatant, amnitotic fluid or cerebrospinal fluid.
This product is only available within the EU!
Method/Platform
lateral flow
Range/Assay Sensivity
50 to 10 000 pg/ml
Test Principle
IL-6 binds to a first anti IL-6 antibody conjugated to gold particles. The IL-6 loaded gold particles diffuse through the membrane and overflow the test line.
A second monoclonal antibody, specific for IL-6 is coated on the membrane. The gold particles are bound specifically and become visible as a coloured line. Colour intensity is directly proportional to the concentration of IL-6 in the sample. The conjugated specific antibodies printed as a second line on the membrane captures the rest of gold conjugate.
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20 tests Test for the semi-quantitative evaluation of human Interleukin-6 (IL-6) in serum, plasma, cell culture supernatant, amnitotic fluid or cerebrospinal fluid.
resDNASEQ CHO Residual DNA Quantitation kit (Without DNA Control )
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Product Info
Description
Features of there sDNASEQ CHO Residual DNA Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
Only one Reagent for qPCR;
Only 1.5 hours will be needed for the whole test.
Accurate
Perfect amplification curve, good amplification efficiency and good precision.
Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
Limit of Detection (LOD): 0.01 fg/μL; Limit of Quantification (LOQ): 0.3 fg/μL
The recovery rate of different concentration samples in the linear range is between 70% and 130%.
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1fg/μL, 0.3fg/μL, 0.5fg/μL, 1fg/μL and 3fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentration values of samples with 0.3fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
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Note: Price not include shipment & duty, contact us to get full quote. The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution
Applications
PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Crude DNA, enzymatic reaction solution
Sample amount
<150μl
Recovery
80%
Elution volume
≥15μl
Time per run
≤10 minutes
Liquid carrying volume per column
800µl
Binding yield of column
20µg
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
High recovery efficiency – up to 80% DNA recovery
Fast – isolation can be completed in 10 minutes by column gel method
Low elution volume – elution volume can be as low as 10μl
Efficient – efficient removal of various inhibitory factors
Kit Contents
Contents
D214102
D214103
Purification Times
100 Preps
250 Preps
Buffer GWP
30 ml
60 ml
Buffer DW2
20 ml
50 ml
Elution Buffer
30 ml
60 ml
HiPure DNA Mini Columns I
50
250
2 ml Collection Tubes
50
250
Storage and Stability
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.
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HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
