Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Salmonella spp. are members of the family Enterobacteriaceae. They are Gram-negative, facultatively anaerobic, flagellated, rod-shaped organisms. They are approximately 0.7 to 1.5 µm in diameter and 2 to 5 µm in length and responsible for a large number of cases of foodborne illness throughout the world. Salmonella have circular DNA genomes with a mean length of approximately 4530 kb, although this can vary by up 1000 kb. Salmonella classification is extremely complex, however, the genus is divided into two species: S. enterica and S.bongori. S. enterica is then itself divided into 6 biochemically distinct subspecies and the Salmonella genus is further classified into serovars (serotypes) based on the lipopolysaccharide (O), flagella protein (H), and sometimes the capsular (VI) antigens. There are more than 2500 known serovars and within a serovar there may be strains that differ in virulence.
Salmonella are mainly transmitted by the faecal-oral route. They are carried asymptomatically in the intestines or gall bladder of many animals, being continuously or intermittently shed in the faeces. Humans can become infected if they do not wash their hands after contact with infected animals or animal faeces. In such instances the bacteria adhere to and enter the cells of the intestinal epithelium. The toxins produced by the bacteria can damage and kill the cells that line the intestines, which results in intestinal fluid loss. The bacteria can survive for weeks in a dry environment and far longer in water thus they are frequently present in polluted waters. Salmonella can also be carried latently in the mesenteric lymph nodes or tonsils; these bacteria are not shed, but can become reactivated after stress or immunosuppression. In addition, fomites and vectors can spread Salmonella and vertical transmission occurs in birds, with contamination of the vitalize membrane, albumen and possibly the yolk of eggs. Salmonella spp. can also be transmitted in utero in mammals.
There are two different disease conditions that are distinct to salmonellosis; gastroenteritis and enteric typhoid fever. The gastroenteritis is a nonsystemic infection of the intestinal tract and regional lymph nodes that gives rise to headache, muscle aches, diarrhoea, vomiting, abdominal cramping, chills, fever, nausea and dehydration. In contrast, the enteric typhoid fever is a systemic disease in which the microorganism replicates within the cells of the reticuloendothelial system. The symptoms usually appear 6 to 72 hours after ingesting contaminated food although individuals can be infected with the bacteria without having symptoms. Those with and without symptoms shed the bacteria in their stool and it is important that personal hygiene be maintained at all times.
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Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
>The display can simultaneously display:all main parameters such as cold trap temperature,vacuum degree,sample temperature, and shelf temperature.
>Schematic diagram of equipment operating components,through graphic display to view the working status of each component of the equipment.
>Ithas the functions of cold trap precooling and vacuum pump preheating,and the time can be set by the userin the system. >Display whether the current running status is normal or not by numerical color.
o One-key operation,the operation is extremely simple,no special professional learning and training is required.
o Thermal electromagnetic over-current and short-circuit protection,making the equipment run more safely and stably.
o Active reminder of equipment maintenance time and vacuum pump replacement lubricating oil time.
o Ambient temperature monitoring,the device automatically reminds the alarm if the ambient temperature is too high.
o Optional eutectic point detection device and endpointjudgment detection system make freeze-drying more scientific.
Dryingchamber
· The separate drying chamber reduces the probability of damage caused byimproper handling of the drying chamber due to height and weight.The upper cover is anodized aluminum with anti-corrosion treatment.●Highly transparent plexiglass drying chamber,good light and heat radiation conduction.Reserve 6/12 external valve ports.· 316L stainless steel all black Teflon coating drying chamber,suitable for drying organic solvents and various corrosive samples,reserved for 6/12 external ports.· 316L stainless steel vertical multi-port multi-manifold external rack(The standard configuration is aplexiglass drying chamber,and one ofthe diying chambers can be optional)
Coldtrapandcondensingcoil
· The cold trap cavity and condensation coil are made of 316L stainless steel.Large opening and channel design,the gas can be quickly captured through the condensing coil.· The cavity and condensing coil are equipped with Teflon anti corrosion coating as standard,which can trap organic solvents and various corrosive solvents.· The condensing coil is placed in the cold trap to increase the condensate catchment area,which can effectively prevent external condensation.
Sampleshelves
· 316Lstainless steel stackable sample shelves,capable oflyophilizing machine solvents and various corrosive solvents.Each shelf can be placed and taken freely without manual work.The distance and height ofeach shelfcan be adjusted freely.-All black coating,good heat absorption performance.-Special hole for freeze-drying of centrifuge tubes,which is conducive to the drying of samples in centrifugetube containers.· All-aluminum Teflon surface anti-corrosion treatment electric heating shelf.
Refrigerationsystem
· Danffos compressorCopper plate heat exchanger,the largest area to increase heat dissipation· Unique refrigeration technology,the temperature of the cold trap can be lowered from ambient temperatureto below-80C°within 2 minutes.· Class A temperature sensor with high temperature accuracy and low error.· Multiple detection and alarm system,the refrigeration system pressure is too high or refrigeration failure cantimely alarm and take corresponding measures.
Techinical parameter
ItemNo
ST7003
ST7006
ST9006
Ice condenser capacity
3kg
6kg
6kg
Ice condenser temperature
-70℃
-70℃
-90℃
Cold trap volume
5L
10L
10L
Freeze dried chamber
Bell shape (standard configuration)/Multi manifold type/T-shaped frame type
Sample shelf
φ200mm.Single layer area 0.03m2 with 3-5 layers available
●260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optiona
Vacuum pump
Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types
Oil mist filte
Standard configuration
Vacuum pump connection pipe
Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
Anti-corrosive treatment
Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents.
External valve
6pieces/optional
1-48 pieces/optional
Eutectic point detection system
Support/Optional
Data output analysis
Support/Optional
Powe
0.6kw
1.1kw
1.5kw
Size
420*545*455mm
510*570*500mm
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Freezer Dryer, 3kgs-6kgs water capturer Model: ST7003,ST7006,ST9006
