Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Genomic DNA Extraction Kit (HMW, Magnetic Beads)
Product Info
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Product Info
Genomic DNA Extraction Kit (HMW, Magnetic Beads)
The Genomic DNA Extraction Kit (HMW, Magnetic Beads) provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using Solid Phase Reversible Immobilization (SPRI) magnetic beads. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality genomic DNA isolation with fast magnetic response time and high binding capacity.
Cat.# 50014 Genomic DNA Extraction Kit for Cells (HMW, Magnetic Beads) Cat.# 50015 Genomic DNA Extraction Kit for Blood (HMW, Magnetic Beads) Cat.# 50016 Genomic DNA Extraction Kit for Tissues (HMW, Magnetic Beads)
The extracted HMW genomic DNA size ranges are dependent on the beads resuspension: 50-150 kb by tube tapping and 40-100 kb by tube vortexing. Purified DNA is recovered at high yield and high purity without RNA contamination. The typical purity ratios of A260/A280 are around 1.8-2.0, and A260/A230 are around 2.2-2.5. Purified HMW genomic DNA is suitable for applications such as long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and other general applications.
Features
High molecular weight DNA: 50 kb to 150 kb
High purity
Simple magnetic beads method
No centrifuge needed
No column needed
No vacuum needed
A portion of the extracted genomic DNA samples were loaded on a PFGE gel with a DNA ladder indicated. Sample A: liver tissue; Sample B: intestine tissue; Sample C: whole blood; Sample D: cultured 293T cells.
Cultured Cell samples
Cultured cells are collected and are resuspended in a buffer and then lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in the Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Blood samples
Whole blood is resuspended in the RBC buffer to remove RBC. The remaining leucocytes are lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Tissue samples
Tissues are homogenized and lysed, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Attogene’ s Cylindrospermopsin Lateral Flow Kit can be used to detect Cylindrospermopsin in source water samples.
Format: Rapid-Water – Run Time: 30 Minutes, enough to run two samples (undiluted and a 10 fold dilution) and negative control.
Cylindrospermopsin (CYN) is a potent cyanotoxin synthesized by select species of cyanobacteria, prominently including Cylindrospermopsin raciborskii. It belongs to the tricyclic alkaloid class, exhibiting a molecular weight of approximately 415 Da. Structurally, cylindrospermopsin features an uracil ring fused with a hydantoin moiety, alongside a guanidino group, attributes that render it highly soluble and polar in aqueous environments.
Cylindrospermopsin is notorious for its profound toxicity towards aquatic organisms and its potential threat to human health through exposure via contaminated water and food sources. Consequently, rigorous monitoring protocols are essential in regions prone to cyanobacterial blooms, where cylindrospermopsin can accumulate in freshwater reservoirs and other aquatic habitats. In recognition of these risks, regulatory bodies such as the United States Environmental Protection Agency (EPA) have implemented an action level guideline. As of 2019, EPA 10-day drinking water health advisory for cylindrospermopsin recommended a threshold of 0.7 parts per billion (ppb), or 700 parts per trillion (ppt) for children under the age of six, and 3 parts per billion, 3000 parts per trillion for anyone older, to effectively manage cylindrospermopsin levels. This precautionary measure aims to uphold both environmental sustainability and public health integrity by minimizing exposure risks. The EPA has also drafted a human health recreational water quality criterion to protect human health at 8,000ppt.
The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines such as CHO, E. coli, E1A, HEK293, Vero, NS0 and Baculovirus. For quantification of residual DNA, we recommend using the resDNASEQ Residual DNA Quantitation kit as described in the resDNASEQ Residual DNA Quantitation kit User Guide. To ensure accurate quantitative results, each sample in triplicate and perform a single PCR reaction for each extraction.
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Features of theresDNASEQ E.coli Residual DNA Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation，and the total time of Sample preparation is about 30 minites.
Good Recovery
The good Recovery rate of Sample Preparation.
Easy to store
All components of the Sample Preparation Kit can be stored at room temperature.
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Note: Price not include shipment & duty, contact us to get full quote. The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines. The operation is easier and the higher nucleic acid yield is guaranteed at the same time.
