Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
Bind and elute all RNA irrespective of size or GC content, without bias
No phenol extractions
No Proteinase K treatment
No carrier RNA
Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
Purify high-quality RNA in 15-20 minutes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified exosomes. It allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Exosomes purified using Norgen’s Purification Kits and most other methods
Size of RNA Purified
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields*
Variable depending on specimen
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component
Cat. 58000 (50 preps)
Lysis Buffer A
20 mL
Lysis Additive B
2 mL
Wash Solution A
18 mL
Elution Solution A
6 mL
Mini Spin Columns
50
Collection Tubes
50
Elution Tubes (1.7 mL)
50
Product Insert
1
Other Products
DBCO-C2-alcohol
Product Info
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Product Info
DBCO-C2-alcohol is a linker containing a DBCO moiety and a terminal primary hydroxyl group. DBCO group can react with azides in copper-free Click Chemistry reactions. The hydroxyl can react with a variety of functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-C2-alcohol is a linker containing a DBCO moiety and a terminal primary hydroxyl group. DBCO group can react with azides in copper-free Click Chemistry reactions. The hydroxyl can react with a variety of functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type
Serum, plasma, acellular samples
Sample amount
300μl
Principle
This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
Safe – no phenol chloroform extraction and no use of Trizol reagent
Fast – several samples can be extracted in 60 minutes by column method
Kit Contents
Contents
R662801
R662802
R662803
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagPure Particles N
1.7 ml
3.5 ml
17 ml
Buffer CFL
6 ml
12 ml
60 ml
Buffer CPL
1.8 ml
3.5 ml
20 ml
Buffer MGW1*
30 ml
60 ml
250 ml
Buffer MW2*
12 ml
25 ml
100 ml
RNase Free Water
10 ml
20 ml
60 ml
Storage and Stability
MagPure Particle N should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
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The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
Kit composition(WLRE8208KIT 48T/Pack)
Composition
Content
E buffer
1 mL×2 Tubes
B buffer
150 μL×1Tube
Positive control template
100 μL×1Tube
Reagents
48T
Product features and advantages:
Product features and advantages
Features
Advantages
High sensitivity
1 copies/ml
Strong specificity
Specificity is superior to PCR
Short reaction time
20min
Polymorphic reagent
Liquid, freeze-dried powder, freeze-dried ball
Easy to operate
Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple
Easy to store and transport
It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation
Low requirements on equipment
Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector
Product application:
Application
Ultra-clean laboratory
African Swine Fever Virus Isothermal Detection
Indoor
Operation procedure and process:
Operation procedure and process:
Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.
Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.
Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid
Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control
Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)
Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.
Performence Comparison Data:
No.
TT value
Template concentration
Result determination
1
4.2
10-1
Positive
2
5.5
10-2
Positive
3
10.2
10-3
Positive
4
17.0
10-4
Positive
5
12.0
10-5
Positive
6
–
10-6
Negative
7
–
10-7
Negative
8
Negative control
Negative
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
Our technical support staff is available to answer any questions you may have about the product.
We offer online tutorials and user guides to help you understand the product better.
We provide technical assistance to help troubleshoot any issues you may be having with the product.
We offer a warranty on our products.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Q: What diseases can Livestock Disease Kit detect? A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
Q: How does Livestock Disease Kit work? A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
Q: How long does it take to get results from Livestock Disease Kit? A: Livestock Disease Kit can give a result within 5-20 mins.
Q: What type of sample is suitable for Livestock Disease Kit? A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
Q: Does Livestock Disease Kit require professional equipment? A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine
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Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
