Total workflow solution
All reagents and components provided from start to finish
Rapid protocol with fewer handling steps
For use with dry swabs
Viral inactivation; Preparation, Amplification, Diagnosis
Total chemistry optimisation
Low shipping costs with ambient shipping
Includes our genesig® Real-Time PCR Coronavirus COVID-19 (CE IVD)
The genesig® Real-Time PCR COVID-19 (CE) is CE-IVD marked and intended for in vitro diagnostic use in Europe.
PLEASE NOTE: This is a Professional Use Only product that requires a trained operator in a controlled laboratory environment. It is NOT a lateral flow or a Point of Care device designed to be used by the general public.
Cell Culture Dish 150mm
Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation
Specifications: 35mm. 60mm.100mm.150mm
PRODUCT FEATURES
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation
Specifications: 35mm. 60mm.100mm.150mm
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments, <1000bp(DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, bodyfluids by spin |
| Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation) |
| Sample type | Serum, plasma and other cell-free fluid samples |
| Sample amount | 1-5ml |
| Elution volume | ≥50μl |
| Time per run | ≤70 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 1mg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).
Kit Contents
| Contents | D318203D |
| Purification Times | 50 Preps |
| Buffer ACL | 250 ml |
| Buffer ACB* | 300 ml |
| Buffer DCW1* | 22 ml |
| Buffer DCW2* | 10 ml |
| Proteinase K | 540 mg |
| Protease Dissolve Buffer | 30 ml |
| Carrier RNA | 110 μg |
| Nuclease Free Water | 20 ml |
| HiPure CFDNA Mini Columns | 50 |
| 2 ml Collection Tubes | 100 |
| Extender Tubes | 50 |
| Sealing O-Ringes | 50 |
| 50ml Collection Tube | 50 |
| Support Tube | 50 |
Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments,
Propargyl-PEG9-alcohol is a crosslinker that can participate in copper catalyzed azide-alkyne Click Chemistry reactions to form stable triazole linkage. The PEG spacer increases the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG9-alcohol is a crosslinker that can participate in copper catalyzed azide-alkyne Click Chemistry reactions to form stable triazole linkage. The PEG spacer increases the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
