Flex Temperature Module Caddy and Calibration Adapter

t-Boc-N-Amido-PEG6-propargyl can be used in copper catalyzed Click Chemistry reactions with azides. The Boc group can be removed under mild acidic conditions to yield the free amine. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

t-Boc-N-Amido-PEG6-propargyl can be used in copper catalyzed Click Chemistry reactions with azides. The Boc group can be removed under mild acidic conditions to yield the free amine. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Description

Attogene’s 0.5mL Micro Spin Desalting Columns are convenient, simple, and ready to be used out of the box. We provide a product that facilitates equal, if not superior results to leading brands products at a significantly better cost. Superlative recovery of proteins and other macromolecules (>7000 MW) with greater than 95% retention of salts, and other small molecules (<1000 MW), are possible even with very dilute (25 ug/mL) samples. Our columns, which are constructed with a simple break away tab at the bottom, are comprised of polypropylene and contain our own proprietary resin slurry that we’ve developed in house to achieve optimal results. Sample volumes between 30-130uL can be loaded while still achieving expected purification numbers.

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Rapid, simple, and reliable components for purification of protein samples
Cleanup before or after antibody biotinylation
Made by Attogene in the USA

Overview

• Simultaneous concentration, desalting and buffer exchange of total urinary proteins
• No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.

ProteoSpin™ Urine Protein Concentration Micro Kit

Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.

ProteoSpin™ Urine Protein Concentration Midi Kit

The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.

ProteoSpin™ Urine Protein Concentration Maxi Kit

The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.

Details

Supporting Data

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Kit Specifications
Minimum Urine Volume Input	1 mL
Maximum Urine Volume Input	5 mL
Maximum Recovered Proteins (5 mL Urine)	3 mg*
Elution Volume	200 – 1000 μL
Time to Complete 10 Purifications	30 minutes

 *Yield will depend on the health status of the donor.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17400 (25 preps)	Cat. 52300 (10 preps)	Cat. 21600 (4 preps)
Wash Solution C	60 mL	60 mL	130 mL
Binding Buffer A	4 mL	4 mL	8 mL
Elution Buffer C	8 mL	30 mL	30 mL
Protein Neutralizer	4 mL	4 mL	4 mL
Micro Spin Columns	25	–	–
Midi Spin Columns (assembled with collection tubes)	–	10	–
Maxi Spin Columns (assembled with collection tubes)	–	–	4
Collection Tubes	25	–	–
Elution Tubes (1.7 mL)	25	–	–
Midi Elution Tubes	–	10	–
Elution Tubes (50 mL)	–	–	4
Product Insert	1	1	1