Flex Temperature Module Caddy and Calibration Adapter
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The Flex caddy allows the Temperature Module GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Temperature Module. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Temperature Module. The module is available with or without the Flex Caddy and Calibration tool: Temperature Module GEN2
Detail
The Flex caddy allows the Temperature Module GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Temperature Module. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Temperature Module. The module is available with or without the Flex Caddy and Calibration tool: Temperature Module GEN2
Other Products
R5211 HiPure DNA/RNA/Protein Kit
Product Info
Document
Product Info
Introduction
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample)
Applications
SDS-PAGE electrophoresis and western blot, etc
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples
Sample amount
Cultured cells: < 10^7Animal tissue: ≤ 20 mgPlant samples: ≤ 150 mgYeast cells: 2 x 10^6 – 5 x 10^7
Principle
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
Advantages
High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
Fast – column method can complete the extraction of several samples in 30 minutes
Safe – no phenol chloroform extraction required
Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
Contents
R521102
R521103
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RLC
50 ml
200 ml
Buffer GW1*
22 ml
66 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
50 ml
3 x 50 ml
RNase Free Water
10 ml
30 ml
Elution Buffer
10 ml
30 ml
Buffer ALO(5%SDS)
10 ml
30 ml
Storage and Stability
HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Document
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Opentrons OT-2 Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN1 P10 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic PipettesOpentrons platform and workstation accuracy and precision performance is not validated with use of non-Opentrons tips.
Document
Opentrons OT-2 Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN1 P10 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic PipettesOpentrons platform and workstation accuracy and precision performance is not validated with use of non-Opentrons tips.
This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus granulosus (and Echinococcus multilocularis) in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Echinococcus granulosus Antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
Document
12 x 8 strips (96 tests)
This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus granulosus (and Echinococcus multilocularis) in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
